| Alu-LTR PCR方法检测HAART治疗系列各细胞亚群的整合型HIV-1 DNA |
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| 引用本文： | 李娟,焦艳梅,高艳青,寇卜心,张彤,吴昊.Alu-LTR PCR方法检测HAART治疗系列各细胞亚群的整合型HIV-1 DNA[J].首都医学院学报,2011,32(4):449-452. |
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| 作者姓名： | 李娟 焦艳梅 高艳青 寇卜心 张彤 吴昊 |
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| 作者单位： | 李娟 (首都医科大学附属北京佑安医院性病艾滋病临床诊疗中心,北京,100069) ; 焦艳梅 (首都医科大学附属北京佑安医院性病艾滋病临床诊疗中心,北京,100069) ; 高艳青 (首都医科大学附属北京佑安医院性病艾滋病临床诊疗中心,北京,100069) ; 寇卜心 (首都医科大学附属北京佑安医院性病艾滋病临床诊疗中心,北京,100069) ; 张彤 (首都医科大学附属北京佑安医院性病艾滋病临床诊疗中心,北京,100069) ; 吴昊 (首都医科大学附属北京佑安医院性病艾滋病临床诊疗中心,北京,100069) ; |
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| 基金项目： | 国家自然科学基金资助项目,北京市属高等学校人才强教计划资助项目(PHR201007112).This,study,was,supported,by,the,National,Natural,Science,Foundation,of,China,Funding,Project,for,Academic,Human,Resources,Development,in,Institutions,of,Higher,Learning,Under,the,Jurisdiction,of,Beijing,Municipality |
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| 摘 要： | 目的应用Alu-LTR PCR方法对高效抗反转录病毒治疗(highly active antiretroviral therapy,HAART)系列的CD4+T,CD8+T及B细胞进行检测,明确不同细胞亚群及HAART治疗的不同阶段是否存在整合的HIV-1 DNA。方法收集20例HIV-1感染患者HAART治疗过程中0、4、12周及10例健康对照的抗凝血标本,纯化CD4+T,CD8+T及B细胞并提取DNA,应用Alu-LTR PCR方法进行检测。结果 20例HIV-1感染者HAART治疗系列中CD4+T细胞及CD8+T细胞成功扩出目的片段,CD8+T细胞所扩条带亮度均低于CD4+T细胞,HAART治疗系列0、4、12周标本所扩条带亮度没有明显差异。B细胞及10例健康者均为阴性。结论 CD4+T及CD8+T细胞存在整合型HIV-1 DNA,HIV储藏库主要存在于CD4+T细胞内。B细胞内不存在整合型HIV-1 DNA。随着HAART治疗的进程,整合型HIV-1 DNA仍然存在,进一步证明HAART不能根除HIV储藏库。
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| 关 键 词： | Alu-LTR PCR 整合型人类免疫缺陷病毒-1 CD4+ T细胞 CD8+ T细胞 B细胞 |
| 收稿时间： | 2011-02-25 |
Optimization of Alu-LTR PCR approach to detect integrated HIV-1 DNA in variety of cell subsets at different stages after HAART |
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| LI Juan,JIAO Yan-mei,GAO Yan-qing,KOU Bu-xin,ZHANG Tong,WU Hao.Optimization of Alu-LTR PCR approach to detect integrated HIV-1 DNA in variety of cell subsets at different stages after HAART[J].Journal of Capital University of Medical Sciences,2011,32(4):449-452. |
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| Authors: | LI Juan JIAO Yan-mei GAO Yan-qing KOU Bu-xin ZHANG Tong WU Hao |
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| Institution: | Clinical Center of STDS and AIDS, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China |
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| Abstract: | Objective To determine if there is integrated human immunodeficiency virus-1(HIV-1) DNA in variety of cell subsets at different stages after highly active antiretroviral therapy(HAART) through detecting HIV-1 DNA in CD4+T cell, CD8+T cell and B cell by Alu-long terminal repeat(LTR) polymerase chain reaction(PCR). Methods The genomic DNA from purified CD4+T, CD8+T, B cells of 20 HIV-1 patients after HAART 0, 4, 12 W and 10 healthy control subjects was obtained by Alu-LTR PCR. Results After Alu-LTR PCR, integrated HIV-1 was detected in each of the 20 cases of HIV/AIDS of CD4+T cells and CD8+T cells after HAART. The product of CD8+T cells were lower than that of the CD4+T cells. The product of HAART 4, 0, 12 W had no significant difference. Conclusion There exists integrated HIV-1 DNA in CD4+T cells and CD8+T cells, and HIV viral reservoir mainly exists in CD4+T cells. Integrated HIV-1 DNA do not exist in B cells. Integrated HIV-1 DNA still exist, and HAART do not eradicate HIV viral reservoir. |
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| Keywords: | Alu-LTR PCR integrated human immunodeficiency virus-1 CD4+T cell" target="_blank">+T cell')" href="#">CD4+T cell CD8+T cell" target="_blank">+T cell')" href="#">CD8+T cell B cell |
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