慢性丙型肝炎患者DNT细胞及T细胞亚群的研究

目的探讨外周血中CD3+CD4-CD8-(double negative T,DNT)细胞及T淋巴细胞亚群在慢性丙型肝炎(chronichepatitis C,CHC)患者中的表达及意义。方法利用流式细胞仪对92例CHC患者、93例正常健康人外周血中的DNT细胞及T细胞亚群进行检测,并对比分析两者的差异。结果 CHC患者DNT细胞百分比(8.57%±6.16%)高于正常对照组(6.78%±4.50%)(P<0.05),其绝对数在两者间比较,差异无统计学意义(P>0.05)。CHC患者CD4+%,CD4+/CD8+比值低于正常对照组(P值均<0.001),CD8+%高于正常对照组(P<0.001)。结论 CHC患者DNT细胞百分比显著升高及T淋巴细胞亚群比例改变,可能是丙型肝炎病毒感染慢性化的重要原因之一。

Double negative T cells and lymphocyte subtype in chronic hepatitis C

Objective To investigate the expression and significance of CD3⁺CD4^-CD8^-(double negative T, DNT) cells and T lymphocyte subsets in chronic hepatitis C cases. Methods DNT cells and lymphocyte subsets of peripheral blood were detected by flow cytometry in 92 chronic hepatitis C patients and 93 healthy people, the differences between them was comparatively analyzed. Results The percent of DNT cells(8.57%±6.16%) were higher in patients with chronic hepatitis C than those in normal control group(6.78%±4.50%)(P<0.05), However, there was no significant difference in the absolute value of DNT cells between them(P>0.05). The CD4 cells, the ratio of CD4⁺/CD8⁺ were decreased but CD8 cells increased in comparison with normal control group(P<0.001). Conclusion The significant increase of DNT cells and the change of T lymphocyte subsets's percentage in chronic hepatitis C patients might be one of the important reason for chronicity and long-term liver damage of hepatitis C.