APP-11肽对糖尿病大鼠肾脏的保护作用

为研究APP 1 1肽对糖尿病 (DM)大鼠肾脏病变的保护作用 ,用链脲菌素诱发大鼠糖尿病 ,皮下注射APP 1 1肽 4周 ,进行血尿素氮、肌酐、2 4h尿白蛋白测定 ,并取肾脏皮质切片进行电镜观察。结果 ,DM大鼠血糖升高 ,胰岛素降低 ,血尿素氮增加 ,尿白蛋白排出量明显增加。电镜显示肾小球基底膜增厚 ,内皮细胞孔增大 ,足突断裂融合。应用APP 1 1肽组虽然未见血糖及其调节激素的改变 ,但尿素氮和尿白蛋白排出量明显减少 ;肾皮质超微结构趋于正常。结果提示APP 1 1肽对DM肾病具有明显改善作用 ,但有关机制还有待进一步研究。

Protective Effect of APP-11 Mel Peptide on Kidney in Diabetic Rats

To observe the protective effect of D-Amyloid precursor protein 63～73 named APP-11 mer peptide from diabetic nephropathy, the peptide was administrated by subcutaneous injection for 4 weeks to diabetic rats induced by streptozotocine. The concentration of glucose and its regulated hormones, urea nitrogen and creatinine in the blood were determined at the end of experiment for 3 groups, hade normal group, diabetic group and diabetic group treated by APP-11 mer peptide. The difference of excretion of uric albumin in the above mentioned 3 groups was also detected, and the kidney cortex was taken for ultrastructure research. The results showed that diabetic rats had much more excretion of uric albumin than that of normal rats and diabetic rats treated by APP-11 mer peptide. Although no effect was observed on the concentration of blood sugar, insulin, glucagon and creatinine, urea nitrogen was decreased in the rats treated by APP-11 mer peptide. On the ultrastructure, thickness of basic membrane and enlargement of the endothelial hole of glomerulus in diabetic kidney were found, but APP-11 mel peptide could improve their changes significantly. The results showed that APP-11 mer peptide can improve the diabetic nephropathy, although no one knows the mechanism.