小鼠大脑中动脉栓塞脑局部缺血模型及其评价方法

目的通过比较客观和可靠的神经行为学评分,以及脑梗死体积和水肿率的计算,评价小鼠大脑中动脉阻塞(middlecerebral artery occlusion,MCAO)局部缺血模型的成功率和可靠性。方法线栓法致小鼠MCAO制备大脑局部缺血模型,应用2,3,5-氯化三苯基四氮唑(TTC)染色和神经行为学评分等方法,计算脑梗死体积、水肿率并观察行为学指标。结果TTC染色证实,MCAO可诱发明显的小鼠脑局灶性缺血,手术成功率约为90.8%,其中以皮质缺血为主,少数为单纯海马和丘脑缺血;脑局部缺血损伤可引起小鼠神经行为学的显著改变,即总体神经行为学评分增加;同时,脑梗死体积约占全脑体积的35%,分析结果准确和稳定。结论本研究成功地建立了MCAO致小鼠脑局部缺血的模型,且有较高的成功率(90.8%);同时所用评价神经功能损伤和脑梗死体积方法具有较高的可靠性。

Evaluation of Middle Cerebral Artery Occlusion Induced Focal Cerebral Ischemia Model in Mice

Objective To study the success rate and reliability of middle cerebral artery occlusion(MCAO) model by objective and reliable neurological score, infarct volume, and edema rate. Methods Cerebral ischemia model of MCAO was established in mouse using the suture method. Infarct volume, edema rate and behavioral performance was evaluated by 2,3,5-triphenyltetrazolium chloride(TTC) staining and neurological score. Results MCAO could induce obvious focal cerebral ischemia by TTC staining and the success rate of this model was about 90.8%. Besides, MCAO could induce three types of cerebral ischemia: cortical, hippocampal and thalamic ischemia. And the predominant type is cortical ischemia. MCAO-induced cerebral ischemia could significantly increase the neurological score compared with the sham group. The procedure for infarct volume evaluation of the MCAO model was accurate and stable; infarct volume was about 35% of entire brain volume. Conclusion Cerebral ischemia model of MCAO was established in mice successfully(90.8%). The evaluation of neurological deficit and infarct volume was technically reliable.