梅毒患者外周血单核细胞体外诱导树突状细胞的形态学与功能研究

目的探讨梅毒患者外周血单个核细胞(PBMC)向树突状细胞(DC)分化的形态学与功能特征。方法分别分离健康人(10例,对照组)和梅毒患者(10例,实验组)外周血中PBMC,与粒细胞巨噬细胞集落刺激因子(GM-CSF)、白介素-4(IL-4)共同培养。用倒置显微镜观察细胞形态,用流式细胞仪测定细胞表型,同时进行混合淋巴细胞反应(MLR),观察其刺激同种异体未致敏T淋巴细胞增殖的能力。结果梅毒患者PBMC经GM-CSF与IL-4诱导获得的细胞具有典型的DC形态特征。细胞表面的CD80、CD83、CD86及HLA-DR的表达率分别为77.92%、39.34%、75.78%和95.42%。与对照组相比,CD80表达明显增高(P<0.05)、CD86表达明显降低(P<0.05),CD83及HLA-DR的表达与对照组比较差异无统计学意义(P>0.05)。MLR结果显示,梅毒患者PBMC诱导分化的DC可以刺激同种异体T细胞增殖。结论经GM-CSF、IL-4刺激后,梅毒患者的PBMC可以诱导分化为具有典型形态特征及抗原呈递功能的DC,DC表型的特征可能影响梅毒患者的细胞免疫状态。

Morphology Observation and Function Study on PBMC to DC in Patients with Syphlis

Objective To investigate the differentiated ability of peripheral blood mononuclear cells(PBMC) to dendritic cell(DC) in patients with syphilis.Methods The PBMC isolated from syphilis patients as well as healthy people were cultured in the completed medium containing GM-CSF,IL-4.The morphology of the DCs was observed using inverted microscope.The phenotypes were investigated with flow cytometry method.To perform mixed lymphocyte reaction(MLR),DCs were cultured with allogeneic T cells.Results The expression of CD80,CD83,CD86 and HLA-DR in syphilis patients were(77.92%),(39.34)%,(75.78%) and(95.42%),respectively.The expression level of CD80 was higher than the controlled people while the level of CD86 was lower.There were no significant differences between the syphilis patients and healthy people as to the level of CD83 and HLA-DR.The results of MLR suggest that the PBMC derived from syphilis can stimulate the T cells proliferation just like normal.Conclusion Compared with healthy people,the DC induced from the PBMC of the syphilis patients has characteristic morphology and the function of the presenting antigen is normal.