罗哌卡因对皮肤损伤愈合及GM-CSF表达的影响

摘要：目的  观察罗哌卡因对皮肤损伤小鼠皮肤愈合及粒细胞-巨噬细胞集落刺激因子（GM-CSF）表达的影响。方法  将小鼠随机分为实验组（罗哌卡因）和对照组（生理盐水），两组小鼠创面进行Masson染色，测定GM-CSF浓度和羟脯氨酸水平，记录创面的愈合率和愈合时间。结果  第5天，实验组绿染胶原纤维沉积量较对照组多；第10天，实验组较对照组创缘上皮增生明显。实验组小鼠第5天GM-CSF含量高于对照组（P < 0.05）。实验组小鼠创面愈合时间短于对照组（P <0.05）。实验组大鼠第5天和第10天创面愈合比例高于对照组（P <0.05）。实验组大鼠第5天和第10天创面羟脯氨酸水平高于对照组（P <0.05）。结论  罗哌卡因局部浸润可以促进小鼠全层皮肤损伤的愈合，其机制可能与局部浸润罗哌卡因能够促进GM-CSF的表达有关。

Effect of local infiltration of Ropivacaine on healing of full-thickness skin lesion and expression of GM-CSF in mice

Abstract: Objective To observe the effect of Ropivacaine on the healing of skin and the expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in mice with skin injury. Methods The mice were randomly divided into experimental group (Ropivacaine group) and control group (saline group). The wounds of mice in the two groups were stained by Masson staining. The concentrations of GM-CSF and hydroxyproline were determined. The wound healing rate and healing time were recorded. Results On the 5th day, the amount of green-stained collagen fibers in the experimental group was larger than that in the control group. On the 10th day, hyperplasia of margin epithelium in the experimental group was more obvious than that in the control group. The level of GM-CSF in the experimental group was significantly higher than that in the control group on the 5th day (P < 0.05). The wound healing time in the experimental group was shorter than that in the control group (P < 0.05). The wound healing ratio and the hydroxyproline level of the wound in the experimental group were higher than those in the control group on the 5th and the 10th day (P < 0.05). Conclusions Local infiltration of Ropivacaine can promote the healing of full-thickness skin lesions in mice, and the mechanism may be related to the promotion of GM-CSF expression.