| 益气温阳活血方对慢性心衰大鼠心室重构钙调磷酸神经酶信号通路的影响研究 |
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| 引用本文： | 杨冬花,李家邦.益气温阳活血方对慢性心衰大鼠心室重构钙调磷酸神经酶信号通路的影响研究[J].中国现代医学杂志,2008,18(21):3078-3082. |
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| 作者姓名： | 杨冬花 李家邦 |
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| 作者单位： | 1. 贵阳中医学院第二附属医院,心内科,贵州,贵阳,550003
2. 中南大学湘雅医学院,中西医结合研究所,湖南,长沙,410008 |
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| 基金项目： | 贵州省优秀科技教育人才省长资金项目(黔省专合字2005-308); 贵州省科学技术基金(黔科合J字2007-2139) |
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| 摘 要： | 目的 探讨中药复方益气温阳活血方对慢性心衰大鼠心室重构钙调磷酸神经酶(CaN)信号通路的影响.方法 体内、外试验应用Fura-2/AM比率荧光成像系统检测心肌细胞内钙离子浓度(Ca2+]i);以RT-PCR法以及Western-blot法检测CaN、活化T细胞核因子3(NFAT3)mRNA及蛋白质表达水平在益气温阳活血方及拆方治疗前后的变化.结果 与正常组比较,模型组Ca2+] i、心肌组织及心肌细胞CaN、NFAT3mRNA及蛋白质的表达水平均明显明显升高;益气温阳活血方能够明显减少Ca2+] i、抑制CaN、NFAT3 mR-NA及蛋白质表达水平的升高.结论 CaN信号通路在慢性心衰心室重构机制中起重要作用;益气温阳活血方可能通过抑制CaN信号通路改善心室重构.
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| 关 键 词： | 慢性心衰 心室重构 CaN NFAT3 益气温阳活血方 |
Effects of decoction of Yiqiwenyanghuoxue on calcineruin signal pathway in the myocardial remodeling of rat with chronic heart failure |
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| YANG Dong-hua
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LI Jia-bang.Effects of decoction of Yiqiwenyanghuoxue on calcineruin signal pathway in the myocardial remodeling of rat with chronic heart failure[J].China Journal of Modern Medicine,2008,18(21):3078-3082. |
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| Authors: | YANG Dong-hua LI Jia-bang |
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| Institution: | YANG Dong-hua1,LI Jia-bang2(1.Department of Cardiology,the Second Affiliated Hospital of Guiyang TCM College,Guiyang,Guizhou 550003,P.R.China,2.Deparment of Traditional , Western Medicine,Xiangya Hospital,Central South University,Changsha,Hunan 410008,P.R.China) |
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| Abstract: | Objective To investigate the effects of decoction of Yiqiwenyanghuoxue on calcineruin(CaN) signal pathway in the myocardial remodeling of rat with chronic heart failure(CHF).Methods The model rats and cardiomyocytes with CHF were treated with decoction of Yiqiwenyanghuoxue,then intracellular Ca2 + concentration(Ca2+ i) was determined by Fura-2/AM ratio imaging analysis.CaN and NFAT3 mRNA evaluated with RT-PCR and protein by Western-blot.ResultsIn CHF compared to the control group, Ca2+ i was significantly h... |
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| Keywords: | chronic heart failure myocardial remodeling CaN NFAT3 decoction of Yiqiwenyanghuoxue |
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