HBV-DNA荧光定量PCR检测的临床意义

目的通过与血清标志物(HBV-M),血清ALT的比较,探讨血清乙肝病毒DNA荧光定量PCR检测的临床意义及其与病毒复制程度变化的关系与原因.方法采用FQPCR法对慢性乙肝病人进行血清HBVDNA定量检测并与血清中乙肝标志物及血清ALT进行相关性分析.结果血清HBVDNA水平与HBvM表现模式有关,其水平变化与HBsAg HBeAg有明显正相关关系,血清HBVDNA水平与ALT成负相关.结论HBV-DNA含量与HBeAg有明显关系,抗HBe阳性患者HBV-DNA含量仍高,病毒突变是其重要原因.HBV-DNA FQPCR检测能更准确地反映病毒复制程度,对乙肝的诊断治疗预后意义重大.

Clinical significance of detecting HBV-DNA by fluorescent quantitative PCR

Objective:By comparing with HBV-M and serum ALT,to explore the clinical significance of the serum HBV-DNA fluorescence ration PCR analysis and the relationship and the cause between HBV-DNA and the replication of hepatitis B virus.Methods:serum HBVDNA was detected by FQPCR.Correlated bivariate analysis was used between HBVDNA and HBV-M and ALT.Results:Serum HBVDNA content was related to HBV-M,their changing levels was significantly positively related to HBsAg and HBeAg.The levels of serum HBVDNA was negatively correlated with ALT.Conclusions:HBV-DNA content has evident relation to the HBeAg.HBV-DNA content of the anti-HBe positive infecter is still high,and hepatitis B virus muta-tion is an important causation.HBVDNA-FQPCR detecting can reflect the copy degree of the hepatitis B virus accurately,and it has positively clinical significance for guiding diagnose treatment and prognosis of HBV infecter.