PCR-SSCP分支杆菌菌种初步鉴定方法的建立及其应用

目的：建立一种简便、快速、灵敏和特异的分支杆菌菌种鉴定方法。方法：通过聚合酶链反应（Ｐｏｌｙｍｅｒａｓｅ Ｃｈａｉｎ Ｒｅａｃｔｉｏｎ，ＰＣＲ）－单链构象多态性（Ｓｕｉｎｇｌｅ－ｓｔｒｎｄｅｄ Ｃｏｎｆｏｒｍａｔｉｏｎ Ｐｌｏｙｍｏｒｐｈｉｓｍ，ＳＳＣＰ）方法分析分枝杆菌１６ＳｒＲＮＡ，根据其电泳图谱与标准株的相似性进行菌种初步鉴定。结果：２９种分支杆菌标准株中，除结接分支杆菌、牛结核分支杆菌和卡

DIFFERENTIATION OF MYCOBACTERIAL SPECIES BY PCR-SSCP

Objective: To develop a simple, rapid, sensitive, and specific method for differenciation of mycobacterial species. Methods: Mycobacterial 16S rRNSA sequences were analyzed by PCR-SSCP, then mycobacterial species were differentiated by their characteristic electrophoresis patterns. Results: Of 29 mycobacterial standard strains, M. tuberculosis, M. bovis and M. bovis BCG had similar SSCP profiles, M. kansassi and M. gastri also exhibited the same SSCP profiles, the other mycobacterial standard strains displayed different SSCP profiles. Of 70 clinical isolate identified as M. tuberculosis complex by BACTEC 460, 55 had the same SSCP profiles as M. tuberculosis, 15 exhibited different SSCP profiles from M. tuberculosis. Of 10 clinical isolate identified as M. tuberculosis by traditional method, only one exhibited different SSCP profiles from M. tuberculosis. Conclusions: PCR-SSCP technique might become a simple, rapid and reliable method for identification of mycobacterial species.