不同尺寸三氧化二铁(Fe2O3)纳米粒子的细胞毒性及氧化作用的实验研究

目的 从细胞半数抑制浓度IC50和氧化作用的角度探讨不同粒径大小纳米Fe2O3粒子细胞毒性的差异。方法 将8、13和37nm3个不同尺寸的纳米Fe2O3粒子以不同剂量、作用时间作用于CHL细胞。用活细胞计数法求得各尺寸粒子的IC50，并绘制细胞生长抑制曲线。硫代巴比妥酸法测定丙二醛(MDA)含量，黄嘌呤氧化酶法测定超氧化歧化酶(SOD)活性。结果 3种尺寸的纳米粒子在一定浓度范围内，剂量与细胞抑制率呈现“剂量-反应”关系，超过这一范围后，量效关系消失；在量效相关的浓度范围内。三个尺寸纳米粒子的IC50分别为：IC50(8nm)=279．8585μg/mL，IC50(13nm)=254、739μg／mL，IC50(37nm)=561．237μg／mL；纳米Fe2O3粒子可引起CHL细胞的氧化应激反应。且与作用时间有关。但MDA、SOD值变化与纳米粒子尺寸、作用剂量之间无明显的量效关系。结论不同粒径的纳米Fe2O3粒子在细胞毒性上表现出一定的差异。同时，纳米Fe2O3粒子可引起CHL细胞的氧化应激反应。从时效性分析推论其细胞毒性与氧化性存在一定的关联。

Study on cytotoxicity and oxidative effects of different sizes of hematite (Fe2O3) nanoparticles on CHL cell in vitro

Objective] To investigate the cytotoxicity among different size of hematite nanoparticles by 50% inhibitory concentration (IC50) and oxidative effects. Methods] CHL cell was used in this study. Three sizes of Fe2O3 nanoparticles 8, 13, and 37nm were administered to the cell cultures with different doses and times as experimental groups. The IC50 and growth-inhibitory curves of cells were assayed by cell counting. The concentration of malondialdehyde (MDA) were determined by thiobarbituric acid reactive substance method and the activity of super oxide dismutase (SOD) was determined by xanthine oxidase method. Results] When cells were exposed to three sizes of nanoparticles respectively within a dose range, the cells inhibitory (CI) rate decreased as the dose increasing with a relationship of dose-effect response, which however disappeared beyond the dose range. Within the range of doseeffect as above, we can calculate the IC50 of three sizes of nanoparticles: IC50(8 nm)=279.585 μg/mL, IC50(13 nm)=254.739 μg/mL, IC50(37 nm)=561.237 μg/mL. The hematite nanoparticles could induce the oxidative stress condition in CHL cells in relation to time, but no notable correlation between the index of MDA, SOD and the size and doses. Conclusion] There is difference of cytotoxicity among different sizes of hematite nanoparticles. Meanwhile the hematite nanoparticles could induce oxidative stress condition in CHL cell, which maybe correlative to its cytotoxicity by time and effect relationship.