运动对2型糖尿病大鼠脂联素和 GLUT4基因表达的影响

目的 观察游泳运动对2型糖尿病大鼠肾周脂肪脂联素和骨骼肌GLUT4基因表达的影响。方法 采用小剂量链脲佐菌素加高脂高热量饲料喂养的方法建立Wistar大鼠2型糖尿病模型，给予游泳运动训练8周，检测大鼠肾周脂肪脂联素mRNA和骨骼肌GLUT4mRNA表达的变化以及血糖、胰岛素、血脂等代谢指标。结果 糖尿病模型组肾周脂肪脂联素mRNA和骨骼肌GLUT4 mRNA表达显著降低，分别较正常对照组降低了45％（P〈0．01）和43％（P〈0．01），空腹血清胰岛素，甘油三酯、总胆固醇和游离脂肪酸水平较对照组显著升高：游泳运动组脂联素和骨骼肌GLUT4mRNA的表达较模型组显著升高，分别为模型组的1．39倍（P〈0．05）和1，62倍（P〈0．01），接近对照水平，空腹胰岛素较模型组显著降低（P〈0．01），甘油三酯、总胆固醇和游离脂肪酸水平均显著低于模型组（P〈0.05或P〈0．01）。结论 游泳运动可能通过提高脂肪脂联素和骨骼肌GLUT4 mRNA的表达，改善胰岛素抵抗。

Effect of swim training on expression of adiponectin mRNA and GLUT4 mRNA in type 2 diabetic rats

Objective] To explore the effect of swim training on the expression of adiponectin and GLUT4 mRNA in type 2 diabetic rats.Methods] Swim training was performed for 8 weeks in the type 2 diabetic Wistar rat model,which was induced by the intraperitoneal injection of a small dose of streptozotocin plus high fat high caloric laboratory chow?The expression of adiponectin mRNA in perirenal fat and GLUT4 mRNA in skeletal muscles were assed by RT-PCR and the change of blood glucose,serum insulin,blood lipid were measured.Result] Compared with the control group,the expression of adiponectin mRNA and GLUT4 mRNA were decreased by 45%(P <0.01),43%(P <0.01)respectively,fast serum insulin,serum triglyceride,total cholesterol and FFA were increased significantly in the model group;The expression of adiponectin mRNA and GLUT4 mRNA were increased by 1.39 times and 1.62 times in swimming group as compared with the control group(P <0.05 and P <0.01,respectively).Compared with the control group,fast serum insulin levels was decreased obviously(P<0.01)and serum triglyceride,total cholesterol and FFA were decreased significantly in the training group(P <0.01 or P <0.05).Conclusion] Exercise can improve insulin resistance through increasing the expression of adiponectin mRNA and GLUT4 mRNA in type 2 diabetic rats.