脑溢安对脑出血大鼠脑内p38MAPK信号转导通路的影响

目的研究脑溢安对脑出血大鼠脑内p38丝裂原活化蛋白激酶(Mitogen-activated protein kinase,p38MAPK)信号转导通路的影响,探讨中药脑溢安颗粒(简称脑溢安)对脑出血的保护作用机制.方法采用胶原酶注射建立大鼠脑出血模型,应用免疫共沉淀、激酶反应及Western blot技术检测脑出血大鼠脑内p38MApK活性变化及脑溢安对p38MAPK活性的影响.结果脑出血损伤后1 h p38MAPK活性增强,出血损伤后6 h达高峰,12 h后下降,至24 h p38MAPK活性消失,脑溢安治疗组(简称治疗组)在脑出血损伤后1,6和12h各时间点p38MAPK活性均较模型组减低.结论脑出血大鼠脑内p38MAPK活性增强,脑溢安能抑制脑出血损伤激活的p38MAPK信号转导通路.

Effects of Nao Yi-an on p38 MAPK signal transduction pathway in experimental intracerebral hemorrhagic brain of rats

Objective: To investigate the effect of the traditional Chinese medicine complex Nao Yi-an granule (NYA) on p38MAPK signal transduction pathway in the intracerebral hemorrhagic (ICH) brain of rats and explore the protective mechanisms of NYA on ICH. Methods: ICH rat models were established by stereo-taxically injecting 0.4 U type Ⅶ collagenase into the right globus pallidus(GP).The p38MAPK activity was an-alyzed by immunoprecipitation, kinase assay and Western blot. Results: The p38 activity was increased 1 h after ICH injury, peaked at 6 h, decreased at 12 h, and disappeared at 24 h. The p38 activity in NYA group was lower than in the model group at 1, 6 and 12 h after ICH injury. Conclusions: The p38MAPK activity in the rat's brain after ICH is significantly increased. NYA can inhibit the p38 signaling transduction path-way.