火把花根和泼尼松对大鼠系膜增殖性肾炎的研究

目的应用火把花根（昆明山海棠）及泼尼松对MsPGN大鼠模型干预，并探讨其作用机制。方法将MsPGN大鼠模型，分成模型对照组、模型治疗组（火把花根及泼尼松），并设空白组对照。每1，2及4周末测24h尿蛋白，处死大鼠各5只，留取肾脏，观察肾脏病理并用RT—PCK及免疫组化方法检测肾组织中MCP-1的mRNA及蛋白质表达。结果MsPGN模型组大鼠尿蛋白、肾小球病理及肾组织内MCP-1的mRNA及蛋白质表达较空白组均显著上调（P〈0．01）。火把彳劬圾泼尼松干预后，上述上调指标均被抑制（P〈0．01）。结论火把花根和泼尼松对MsPGN大鼠模型干预的效果较好，此疗效可能与下调肾组织MCP-1相关。两药可能有协同作用。

Study of pathogenesy using Colquhoumia root and Prednisone in MsPGN

Objective] To observe effects of treatment with Colquhoumia root(Tripterygium hypoglaucum(Le,vl)Hutch)and Prednisone in MsPGN,and investigate their pharmacological mechanisms.Methods] Wistar rats were injected ATS to make MsPGN model and then randomly divided into 3 groups:nomal control group,model group,and model +Col+Pre treated group.By 1,2 and 4 weeks,the proternurua level was measured.Histopathological,immunohistochemical,RT-PCR technique were used to evaluate the mesangial matrix production,the mRNA and the protein expression of MCP-1 in the tissue of kidney respectively.Results] The proteinuria and the relative area of glomerular mesangium were increased significantly in model group.The expression of MCP-1 mRNA and protein in their kidney were much higher in model group than control group(P <0.01).After treatment with Col and Pre,the proteinuria,the expression of MCP-1 mRNA and protein in their kidney were significantly decreased in treatment groups as compared with those in model groups.Conclusions] Col and Pre are effective in reducing proteinuria and mesangial matrix proliferation in MsPGN,and it may achieve these effects by inhibiting the expression of MCP-1 mRNA and protein in their kidney.There is synergism between two drugs.