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| 鲍曼不动杆菌产PER-1型ESBLs基因的克隆、测序及分析 | |
| 引用本文： | 李蓉,李文林,石小玉,梁朝,徐小文,赵林.鲍曼不动杆菌产PER-1型ESBLs基因的克隆、测序及分析[J].中国现代医学杂志,2008,18(13). |
| 作者姓名： | 李蓉 李文林 石小玉 梁朝 徐小文 赵林 |
| 作者单位： | 1. 南昌大学医学院,微生物学教研室,江西,南昌,300006 2. 江西省医学生物高技术重点实验室,江西,南昌,330006 3. 江西省南昌市东湖区卫生局,江西,南昌,330006 |
| 摘 要： | 目的分析产PER-1型超广谱β-内酰胺酶（ESBLs）鲍曼不动杆菌的耐药性及其基因的核苷酸序列。方法先后用最低抑菌浓度（MIC）初筛法和纸片扩散确认法从临床分离的鲍曼不动杆菌中检测产ESBLs菌株，并用聚合酶链式反应（PCR）、克隆测序方法分析质粒上PER-1型ESBLs基因。结果93株鲍曼不动杆菌中，产ESBLs菌株有16株，占17．20％，均为多重耐药菌。其中PER-1型ESBLs阳性菌株有8株；TA克隆后测序表明与铜绿假单胞菌（AJ621265．1）blaPER-1基因序列100％同源。结论发现质粒上同时带产TEM-1、PER-1型ESBLs和OXA-23型碳青霉烯酶基因的鲍曼不动杆菌，blaPER-1基因可能来源于铜绿假单胞菌。 |
| 关 键 词： | 鲍曼不动杆菌 超广谱β-内酰胺酶(ESBLs) 基因 鲍曼不动杆菌 ESBLs 酶基因 克隆 测序 分析 Cloning Acinetobacter baumannii encoding gene analysis sequencing 来源 碳青霉烯 发现 基因序列 铜绿假单胞菌 阳性菌株 多重耐药菌 结果 |
Cloning, nucleotide sequencing and analysis of gene encoding a PER-1 ESBLs in Acinetobacter baumannii |
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| LI Rong,LI Wen-lin,SHI Xiao-yu,LIANG Chao,XU Xiao-wen,ZHA Lin.Cloning, nucleotide sequencing and analysis of gene encoding a PER-1 ESBLs in Acinetobacter baumannii[J].China Journal of Modern Medicine,2008,18(13). | |
| Authors: | LI Rong LI Wen-lin SHI Xiao-yu LIANG Chao XU Xiao-wen ZHA Lin |
| Institution: | 1.Microbiology Department;Medical College of Nanchang University;Nanchang;Jiangxi 300006;P.R.China;2.Jiangxi Key Laboratory of Medical Biotechnology;Jiangxi 330006;3.Donghu Board of Health;P.R.China |
| Abstract: | Objective] To analyze drug resistance of Acinetobacter baumannii producing PER-1 ESBLs and its nucleotide sequence. Methods] The ESBLs producing strains were screened by minimal inhibitory concentration(MIC)method and confirmed by a disc dilution confirmatory test. PER-1 ESBLs gene in plasmid was analyzed by PCR, cloning and nucleotide sequencing. Results] There were 16 ESBLs producing strains in 93 isolates of A.baumannii and accounted for 17.20%. They were all multi-drug resistance in which 8 were PER-... |
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