血清色氨酸测定对中枢性疲劳症候群诊断价值的研究

目的 :建立快速、简便、准确的检测血清色氨酸的方法 ,以研究其对中枢性疲劳症候群的诊断价值。方法 :用高效毛细管电泳法检测了 10 0例健康人 ,6 7例临床症状符合中枢性疲劳症候群的患者血清中色氨酸。结果 :线性范围为 5～ 6 40umol/L ,最低检出限为 2 .5umol/L ,平均回收率为 98.35 % ,批内精密度为 5 .8% ,批间精密度为 8.1%。对 10 0例正常对照组与 6 7例中枢性疲劳症候群的患者血清色氨酸测定结果进行统计分析 ,两者差异有显著意义 (P <0 .0 1)。结论 :高效毛细管电泳法快速检测血清色氨酸为中枢性疲劳候群的诊断提供了一种有效的临床实验室依据。

DIAGNOSTIC VALUE OF THE DETERMINATION OF TRYPTOPHAN IN SERUM FOR CENTRAL FATIGUE SYNDROME

Objective:To develop a rapid and accurate assay for the determination of tryptophan in serum, and to determine the diagnostic value for Central Fatigue Syndrome (CFS) by the assay. Methods:We determined tryptophan in serum from 100 healthy individuals and 67 cases of the clinical manifestation according with CFS by high performance capillary electrophoresis (HPCE). Results:The assay was linear from 5 to 640 umol/L, the minimal detection limit was 2.5 umol/L, analytical recovery was 98.35%, the with-run precision was 5.8%, and between-run precision was 8.1%. The tryptophan concentrations in serum of 67 patients with CFS and 100 healthy individuals were determined,and the difference was significant between the two groups ( P <0.01). Conclusions:HPCE has many advantages such as rapidity, simplicity, accuracy and sensitivity,and the method being used for determining tryptophan in serum is important in evaluating the diagnosis of CFS.