微R-181a对人白血病K562细胞株的抑制作用及其机制的初步探讨

目的 探讨miR-181a对白血病K562细胞生长的抑制作用及其机制。方法 利用Oligofectamine脂质体法将化学合成的miR-181a转染K562细胞，采用四甲基偶氮唑蓝（MTT）法和台盼蓝拒染法检测其对K562生长抑制的作用，结合基因芯片技术检测miR-181a对K562细胞基因表达谱的影响，筛选肿瘤相关差异基因。结果 MTT法和台盼蓝拒染法结果显示，转染后各时间点转染组K562细胞的生长均受到了明显地抑制。瑞氏一吉姆萨染色显示，转染组细胞表现出典型的生长抑制的变化。基因芯片筛出肿瘤相关差异基因30个，其中20个表达下调，10个表达上调。结论 miR-181a可能通过增强抑癌基因的表达，削弱癌基因的表达，从而抑制白血病细胞的增殖和生长。因此。其抗癌效应可应用于肿瘤的治疗。

Inhibitory effect of miR-181a on human K562 leukemia cells cell line

Objective] To study the growth inhibitory effect and mechanism of miR-181a on leukemia K562 cells. Methods] We transfected chemically synthesized miR-181a duplex into human K562 leukemia cells using Oligofectamine reagent and the growth inhibitory rate of K562 cell was measured by MTT assay and trypan blue dye exclusion method. Morphologic evidence for inhibition was determined by staining with Wright-Giemsa. The influence of such microRNA upon the gene expression profile was studied by microarray and the tumor-associated genes differentially expressed in transfected group K562 cells were identified using PANTHER classification Systems. Results] MTT assay and trypan blue dye exclusion method showed that transfection of miR-181a remarkably decrease the cell growth at 24, 48 and 72 h post-transfection. Morphological observation after staining with Wright- Giemsa showed that K562 cells transfected with miR-181a revealed characteristic growth inhibitory. We identified 30 tumor-associated genes including 20 down-regulated gene and 10 up-regulated genes. Conclusion ] MiR- 181 a may inhibit the growth and proliferation of the leukemia cells through inhibition of the expression of oncogenes and increase of the expression of tumor suppressor genes. Thus, the anti-cancer effect of miR-181a may be potentially exploited for the cancer therapy.