大鼠胎肝前体细胞的分离培养

目的：探索胚龄(ED)13．5d大鼠胚胎肝脏中肝前体细胞的生物学特性，为细胞移植及基因导向治疗奠定基础。方法：酶消化法分离肝前体细胞，观察不同体外培养对肝前体细胞生物学功能的影响，并用免疫组化方法鉴定分离培养细胞的分化标志。结果：肝前体细胞在原代胚胎成纤维细胞饲养层(PREF)培养，其增殖能力较无饲养层培养强，原代培养可保持未分化状态。细胞分化标志鉴定提示EDl3．5d大鼠胚肝前体细胞中存在双分化能力的细胞。结论：EDl3．5d大鼠胚肝前体细胞存在双分化能力的原始肝细胞，肝前体细胞可在PREF进行体外扩增。

Isolation and Cultivation of Fetal Liver Epithelial Progenitor Cells

Objective To investigate biological characteristic of hepatic progenitor cells from fetal liver of rat on ED13.5 days and establish the foundation for liver cell transplantation. Methods Fetal liver cells were isolated by enzymatic digestion. Different influence on cellular biologic action was observed under various cultured conditions and liver specific markers of cells were detected by immunocytochemistry. Results Fetal liver epithelial progenitor cells proliferated on primary rat embryonic fibroblast (PREF) feeder better than non-feeder and were kept undifferentiation. Cell differentiation marks suggested that fetal liver epithelial progenitor cells contain bipotential cells on ED13.5 d.Conclusion There retains hepatic stem cells in rat fetal liver on ED 13.5 d and PREF can be used for amplification of fetal liver epithelial progenitor cells in vitro.