| 微卫星DNA分析国内24个近交系小鼠遗传状况 |
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| 引用本文： | 李银银,吴绍亮,王洪,萧晓琴,张双悦,郭萌,李长龙,吕建祎,刘欣,陈振文,杜小燕.微卫星DNA分析国内24个近交系小鼠遗传状况[J].中国比较医学杂志,2017,27(8):43-49. |
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| 作者姓名： | 李银银 吴绍亮 王洪 萧晓琴 张双悦 郭萌 李长龙 吕建祎 刘欣 陈振文 杜小燕 |
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| 作者单位： | 首都医科大学基础医学院实验动物学系, 北京 100069,山东省临沭县人民医院, 山东 临沂 276700,中国食品药品检定研究院实验动物资源研究所, 北京 100050,首都医科大学基础医学院遗传学系, 北京 100069,首都医科大学基础医学院遗传学系, 北京 100069,首都医科大学基础医学院实验动物学系, 北京 100069,首都医科大学基础医学院遗传学系, 北京 100069,首都医科大学基础医学院遗传学系, 北京 100069,首都医科大学基础医学院遗传学系, 北京 100069,首都医科大学基础医学院遗传学系, 北京 100069,首都医科大学基础医学院实验动物学系, 北京 100069 |
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| 基金项目： | 国家科技支撑计划（2015BAI07B01）；国家自然科学基金（31372272，31572341）。 |
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| 摘 要： | 目的利用微卫星位点对国内24种近交系小鼠进行遗传状况分析。方法用前期筛选的富含多态性和等位基因数多的30个小鼠微卫星位点,合成荧光标记引物,对近交系小鼠基因组DNA进行PCR的扩增,经STR扫描对各近交系小鼠品系进行基因分型。结果在24个近交系小鼠品系中,有16个品系在品系内在30个位点上均具有相同基因型。而在不同品系间同一位点具有多态性,可初步对不同品系进行鉴别区分。其余品系内个别动物存在多态性。结论所选位点可以参考用于近交系小鼠遗传质量检测分析,并进行初步品系检测鉴定。
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| 关 键 词： | 近交系小鼠 遗传质量分析 微卫星DNA |
| 收稿时间： | 2016/11/29 0:00:00 |
Genetic quality analysis of 24 domestic inbred mouse strains by microsatellite DNA |
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| LI Yin-yin,WU Shao-liang,WANG Hong,XIAO Xiao-qin,ZHANG Shuang-yue,GUO Meng,LI Chang-long,LV Jian-yi,LIU Xin,CHEN Zhen-wen and DU Xiao-yan.Genetic quality analysis of 24 domestic inbred mouse strains by microsatellite DNA[J].Chinese Journal of Comparative Medicine,2017,27(8):43-49. |
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| Authors: | LI Yin-yin WU Shao-liang WANG Hong XIAO Xiao-qin ZHANG Shuang-yue GUO Meng LI Chang-long LV Jian-yi LIU Xin CHEN Zhen-wen and DU Xiao-yan |
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| Institution: | Department of Laboratory Animal, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China,People''s Hospital of Linshu County, Shangdong Linyi 276700,Institute of Laboratory Animal Resources, National Insitute for Food and Drug Control, Beijing 100050,Department of Genetics, School of Basic Medical Sciences, Capital Medical University, Beijing 100069,Department of Genetics, School of Basic Medical Sciences, Capital Medical University, Beijing 100069,Department of Laboratory Animal, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China,Department of Genetics, School of Basic Medical Sciences, Capital Medical University, Beijing 100069,Department of Genetics, School of Basic Medical Sciences, Capital Medical University, Beijing 100069,Department of Genetics, School of Basic Medical Sciences, Capital Medical University, Beijing 100069,Department of Genetics, School of Basic Medical Sciences, Capital Medical University, Beijing 100069 and Department of Laboratory Animal, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China |
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| Abstract: | Objective To analyze the genetic quality of 24 domestic inbred strains mice using microsatellite loci panel. Methods Previously selected 30 microsatellite loci of mouse with high polymorphism and more allele numbers were used to synthesize corresponding fluorescently-labeled primers. Then the genomic DNA samples of each mouse were amplified by PCR and the products were analyzed by STR scanning to genotype the inbred strains of mice. Results Out of the 24 inbred strains, 15 inbred strains showed the same genotype within one strain at 30 loci. Among different strains, microsatellite loci indicated polymorphism which could be used to distinguish different strains. However, the rest 9 strains demonstrated polymorphism within strains. Conclusions Our stuoly provides a useful microsatellite panel to detect genetic quality of inbred mice and distinguish different strains with the optimized microsatellite loci. |
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| Keywords: | Inbred strains Mice Genetic quality Microsatellite DNA |
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