食蟹猴体内、外感染SIVmac251后产毒水平的比较研究

目的探索在猴免疫缺陷病毒（SIV）造模之前选择适宜于制作此模型的猕猴个体的方法,以减少感染后血浆病毒水平过低的几率,从而保证实验的质量.方法测定SIV感染前实验猴外周血单个核细胞（PBMC）在试管内感染SIV后的产毒水平,并与该个体在感染同一株SIV后血浆病毒载量的水平进行比较分析,判断此法能否用于选择适于造模的实验猴.结果根据14只食蟹猴的结果,体外与体内的病毒水平符合比为10/14,体内病毒载量偏低的猴6只（6/14）,体外病毒水平偏低者4只,如把这4只猴排除,则余下10只猴体内病毒载量偏低的猴为3只（3/10）.结论应用检测体外PBMC病毒产量水平的方法,选择病毒水平高的猴才用于体内感染SIV.这样能部分去除体内感染的病毒载量偏低的猴.但最终的解决方法仍是建立培育遗传背景清楚的SPF实验猴群.

A Comparison Study between the SIVmac251 Titer in Cultured PBMC and the Plasma Viral Load after Infection

Objective To explore a method which is being used to screen applicable monkeys for the SIV modeling.Method The pre-infection monkey's PBMCs were cultured and infected with SIVmac251,then,the viral level was tittered and compared with the plasma viral load from the same monkey which infected with the same SIVmac251.The results were analyzed and evaluated whether it may be used to screen the applicable monkeys for SIV model.Result According to the data of 14 monkeys both in vitro and in vivo,the coincidence rate was 71.43%.There were 6 monkeys(42.9 %) with lower viral load in vivo and 4 monkeys with lower viral titer in vitro.If these 4 monkeys with lower viral titer were ruled out,there remained 10 monkeys,and there were only 3 monkeys(30%) with lower viral load among them.Conclusion By using of the method to detect PBMC viral titer in vitro,it might be helpful to choose the higher titer monkeys for modelling SIV infection efficiently.However,it isn't a perfect method.Establishing the clear genetic file of SPF monkey colony may be a reasonable way to get satisfactory monkey model for research use.