野生来源TW近交系小鼠的遗传监测

目的　对中国特有野生来源的TW (TianjinWild)小鼠近交系进行遗传质量检测。方法　按照国标GB T14 92 7 1 2 0 0 1、GB T14 92 7 2 2 0 0 1及WHO(国际实验动物科学理事会 )遗传检测操作规程 ,对TW近交系小鼠进行Akp1等 2 5个遗传生化标记基因纯合度测定 ,进行组织相容性基因纯合度的测定。结果　对TW近交系小鼠进行Akp1等 2 5个遗传生化标记基因的测定 ,所检基因均为纯合体。同时 ,在所检的 2 5个遗传位点中 ,发现有 8个罕见生化标记基因多态性位点 ;检测的组织相容性基因为纯合。结论　按照国家标准 ,野生来源的TW小鼠近交系遗传纯合度已达国标 ,成为高度纯合的近交实验小鼠品系 ,并可望成为具有标准基因库 (型 )的近交小鼠品系

Genetic Monitoring to Wild Derived TW Inbreeding Mouse

Objective To monitor the genetic quality of the new wild derived inbred strain of mice trapped originally in China. Methods According to national standard, GB/T14927 1-2001, GB/T14927 2-2001 and the procedure from International Laboratory Animal Commission, 25 biochemical markers for genetic monitoring of TW (Tianjin Wild) inbred strain were performed, and skin grafting test was also carried out. Results Measurement of 25 biochemical markers gene loci was performed by electrophoresis and the results revealed that all observed gene loci were homogenous without any polymorphism and mutation. Of the 25 loci observed, 8 were found to show the rare subtypes. The skin grafting test showed that no dropping graft was found during 100 days after transplantion, implying the TW was of isohistogenicity. Conclusion\ The TW inbred strain mouse is high homogenous in the genetic characteristics and it is possible for the TW inbred strain to be a standard model of genetic monitoring of biochemical markers.