| 甲型H1N1流感病毒感染不同免疫缺陷小鼠的比较 |
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| 引用本文： | 刘颖,邓巍,徐艳峰,朱华,马春梅,李枫棣,鲍琳琳,秦川.甲型H1N1流感病毒感染不同免疫缺陷小鼠的比较[J].中国比较医学杂志,2011,21(12):1-4,17,93. |
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| 作者姓名： | 刘颖 邓巍 徐艳峰 朱华 马春梅 李枫棣 鲍琳琳 秦川 |
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| 作者单位： | 中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室;中国医学科学院医学实验动物研究所,国家中医药管理局人类疾病动物模型三级实验室 |
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| 基金项目： | 中国医学科学院医学实验动物研究所基本科研业务费，北京市科委项目，中医药行业科研专项 |
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| 摘 要： | 目的宿主免疫系统的功能状态在病毒的感染中起着至关重要的作用,本实验观察了不同免疫缺陷小鼠感染甲型H1N1流感病毒的差异。方法使用六个品系的近交系小鼠,经乙醚麻醉后进行滴鼻攻毒,分析其在病毒感染后存活率、体重变化和肺组织病理改变的异同。结果感染H1N1病毒的6种小鼠在观察的14 d内,野生型的C57BL/6小鼠感染开始体重缓慢下降,感染后期有所回升,有半数存活;BALB/c小鼠和四种免疫缺陷品系小鼠感染病毒后体重随病情发展快速下降,死亡率均为100%。野生型C57BL/6小鼠感染初期为较弥漫的间质性肺炎,后期病变逐渐局限;BALB/c小鼠和四种免疫缺陷品系小鼠感染病毒后出现弥漫的中重度间质性肺炎,细支气管上皮有变性坏死,但炎症细胞明显少于C57BL/6小鼠。结论在甲型H1N1流感病毒的初次感染中固有免疫和特异性免疫分别在感染的初期和后期起主要作用,宿主免疫系统的功能状态影响着甲型H1N1病毒感染和预后。
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| 关 键 词： | H1N1甲型流感病毒 感染 免疫缺陷 Scid小鼠 NOD/LtJ小鼠 BALB/c-nu小鼠 NOD-Scid小鼠 |
Comparison of Different Immunodeficient Mouse Strains Infected with Influenza A(H1N1)Virus |
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| LIU Ying,DENG Wei,XU Yan-feng,ZHU Hua,MA Chun-mei,LI Feng-di,BAO Lin-lin,QIN Chuan.Comparison of Different Immunodeficient Mouse Strains Infected with Influenza A(H1N1)Virus[J].Chinese Journal of Comparative Medicine,2011,21(12):1-4,17,93. |
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| Authors: | LIU Ying DENG Wei XU Yan-feng ZHU Hua MA Chun-mei LI Feng-di BAO Lin-lin QIN Chuan |
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| Institution: | LIU Ying,DENG Wei,XU Yan-feng,ZHU Hua,MA Chun-mei,LI Feng-di,BAO Lin-lin,QIN Chuan(Key Laboratory of Human Disease Comparative Medicine,Ministry of Health,Key Laboratory of Human Disease Animal Models,State Administration of Traditional Chinese Medicine,Institute of Laboratory Animal Science,CAMS & PUMC,Beijing 100021,China) |
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| Abstract: | Objective The functional status of the host immune system plays an important role in viral infection.The aim of this work was to study the differences among four immunodeficiency strains of mice and two wide-type mouse strains after infection of influenza A(H1N1)(A/California/7/2009(CA7)) virus.Methods The six inbred laboratory strains of mice were anesthetized by inhalation of ether and inoculated intranasally with 0.05 mL of infectious virus H1N1(CA7) suspension.The body weight loss kinetics,survival rate... |
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| Keywords: | Influenza A(H1N1) virus infection Immune deficiency SCID mouse NOD/LtJ mouse BALB/c-nu mouse NOD-SCID mouse |
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