关木通致SD大鼠肾脏损害的实验研究

目的　通过动物实验研究关木通对肾脏的毒性作用。方法　将SD大鼠随机分成 3组 ,即对照组及关木通S组和关木通B组 ,关木通两组每天灌胃不同浓度的水煎液 ,对照组灌胃自来水。 7d后测尿量、尿糖、尿蛋白及血肌酐并处死关木通B组大鼠 ,取肾脏做病理。于 30d、6 0d测对照组和关木通S组大鼠尿量、尿糖、尿蛋白及血肌酐、尿素氮 ,并处死 10只S组大鼠取肾脏做病理。 90d时重复上面的实验并处死对照组和关木通S组动物。结果　关木通B组大鼠死亡 2只 ,病理显示肾小管弥漫性坏死 ;关木通S组与对照组比较差异无显著性 ,P >0 0 5。结论　大剂量关木通中毒导致的肾损害为急性肾小管坏死 ,小剂量关木通也不宜长期应用。

Toxicity of Caulis Aristolochiae Mansshuriensis on the kidney of SD Rats

Objective To investigate the toxicity of Caulis Aristolochiae Mansshuriensis(CAM).Methods 50 SD rats were divided into three groups:S and B groups which were given daily dose of 6g and 1g CAM in 2ml respectively,and control group,which fed with 2ml water.At 7 day after,urinary volume,urinary sugar,urinary protein,blood creatinine were measured,and B group was sacrificed for kidney pathological examination.At one and two month after,those of S and control groups were measured again.Of S group,10 rats were sacrificed for kidney check.At 3 month after,those did again,and all of them were sacrificed.Results 2 rats of B group died showing the kidney necrosis.The difference between S and control groups was of no significance (P>0.05).Conclusion High dose of CAM damaged the kidney,resulting in acute renal tubules necrosis.;