首页 | 本学科首页   官方微博 | 高级检索  
检索        

巴马小型猪大肠埃希菌的分离鉴定及耐药性试验
引用本文:陈克研,张贺,陈振文,王洋,王承利.巴马小型猪大肠埃希菌的分离鉴定及耐药性试验[J].中国比较医学杂志,2012,22(7):40-43.
作者姓名:陈克研  张贺  陈振文  王洋  王承利
作者单位:沈阳军区总医院实验动物科;首都医科大学基础医学院实验动物学系
摘    要:目的分离鉴定引起巴马小型猪腹泻的细菌并筛选出对其敏感的特效药物。方法通过常规细菌学检测、分子生物学鉴定、生化试验等方法对疑似细菌感染的巴马小型猪进行分析,同时选用呋喃妥因、美满霉素、庆大霉素等25种药物对分离的细菌进行药敏实验。结果通过以上实验判定巴马小型猪为大肠埃希菌感染引起的腹泻,血清型为O138;药敏试验分析结果表明,该菌株仅对青霉素、链霉素、头孢曲松和呋喃妥因4种药物敏感,对磷霉素、磺胺脒、左氟沙星等高度耐药。结论大肠埃希菌广泛存在耐药性,该研究结果为防治相关实验动物大肠埃希菌感染提供理论依据。

关 键 词:巴马小型猪  大肠埃希菌  分离鉴定  耐药性

Isolation, Identification and Resistance of Colibacillus of Bama Minipigs
CHEN Ke-yan,CHEN Zhen-wen,ZHANG He,WANG Yang and WANG Cheng-li.Isolation, Identification and Resistance of Colibacillus of Bama Minipigs[J].Chinese Journal of Comparative Medicine,2012,22(7):40-43.
Authors:CHEN Ke-yan  CHEN Zhen-wen  ZHANG He  WANG Yang and WANG Cheng-li
Institution:1(1.Department of Laboratory Animal,General Hospital of Shenyang Military Region,Shenyang 110840,China; 2.Department of Laboratory Animal Science,Capital University of Medical Sciences,Beijing 100069,China)
Abstract:Objective To isolated and identify a bacterium from Bama minipigs of diarrhea and the patent medicine were screened by drug susceptibility test.Methods A bacterium isolated from Bama minipigs was identified by morphological,germiculture,staining,Molecular biology and biochemical characteristics.The resistance test were used to selected the sensitive medicine by 25 drugs.Results The Colibacillus of Bama Minipigs were success isolated and identified as serotype O138.The result of resistance test to 25 drugs showed that the bacterium was sensitive to Penicillin、Streptomycin、Rocephin and Nitrofurantoin,but highly resistant to another drugs that were used.Conclusion The animal regression test showed that the bacterium have high pathogenicity.The result of this paper would provide the theory basis for prevention and control Colibacillus Infections of related experimental animal.
Keywords:Bama minipigs  Colibacillus  Isolation and identification  Resistance
本文献已被 CNKI 等数据库收录!
点击此处可从《中国比较医学杂志》浏览原始摘要信息
点击此处可从《中国比较医学杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号