微卫星技术对近交系小鼠遗传质量的分析

目的对14个品系近交系小鼠24个微卫星座位进行遗传分析,以期用微卫星位点分析法区分不同近交系小鼠。方法通过Mouse Genome Informatics数据库确定合适的微卫星位点和引物,对近交系小鼠基因组DNA进行扩增,分析不同品系小鼠在所选微卫星座位的基因片段,与数据库小鼠品系数据进行比较,并对14个品系近交小鼠进行了DNA多态性分析。结果24个微卫星位点在不同品系的小鼠之间具有多态性,不同品系小鼠之间的遗传距离为0.045~1.526;在不同的亚系之间也有具有多态性。结论微卫星标记方法能区分不同的近交小鼠品系、近交小鼠亚系,为小鼠遗传质量的检测提供了一个快捷简便的方法。

Microsatellite Markers for Analysis the Genetic Polymophism of Inbred Mice

Objective To analyze the genetic polymorphism of 14 inbred mice in 24 microsatellite loci and to establish a method for detecting the genetic quality of inbred mice by using PCR technique.Methods We selected suitable microsatellite loci and primers according to the database of Mouse Genome Informatics to amplify DNA in 14 inbred strains of mice,and analyzed the polymorphism of 14 strains of mice in these 24 microsatellite loci.Result There were remarkable polymorphism in 24 microsatellite loci among 14 inbred strains or sub-strains.The genetic distance between strains was from 0.045 to 1.526.Conclusion The microsatellite markers can be used for distinguishing different mice strains and sub-strains.So we should use microsatellite markers to monitor the genetic quality of the inbred mice.