| 树鼩鞭毛虫的形态学观察及其18S rRNA基因分析 |
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| 引用本文： | 陈玲霞,尹博文,李振宇,孙晓梅,代解杰.树鼩鞭毛虫的形态学观察及其18S rRNA基因分析[J].中国比较医学杂志,2015,25(11):5-8. |
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| 作者姓名： | 陈玲霞 尹博文 李振宇 孙晓梅 代解杰 |
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| 作者单位： | 中国医学科学院/北京协和医学院医学生物学研究所树鼩种质资源中心, 云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院/北京协和医学院医学生物学研究所树鼩种质资源中心, 云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院/北京协和医学院医学生物学研究所树鼩种质资源中心, 云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院/北京协和医学院医学生物学研究所树鼩种质资源中心, 云南省重大传染病疫苗研发重点实验室, 昆明 650118;中国医学科学院/北京协和医学院医学生物学研究所树鼩种质资源中心, 云南省重大传染病疫苗研发重点实验室, 昆明 650118 |
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| 基金项目： | 国家科技支撑计划项目(2014BAI01B01);云南省科技创新平台建设(2013DA002)。 |
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| 摘 要： | 目的 对树鼩体内的鞭毛虫进行形态学观察及基因分析鉴定。方法 取树鼩回盲部内容物和粪便经过滤离心处理直接涂片和碘液染色镜检,传统方法提取虫体总DNA,PCR扩增该鞭毛虫的18S rRNA,测序后经BLAST进行同源性分析,并应用MEGA5.1绘制系统发育进化树。结果 形态学观察表明树鼩体内的鞭毛虫为三毛滴虫;测序分析表明树鼩鞭毛虫序列18S rRNA与已报道的胎儿三毛滴虫18S rRNA(AY754332.1)同源性高达99%。结论 树鼩体内可感染胎儿三毛滴虫,研究结果为树鼩的寄生虫学质量控制提供依据。
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| 关 键 词： | 鞭毛虫 树鼩 形态观察 18S rRNA |
| 修稿时间： | 2015/9/18 0:00:00 |
Morphological observation and analysis of its 18S rRNA gene of flagellate in tree shrews |
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| CHEN Ling-xi,YIN Bo-wen,LI Zhen-yu,SUN Xiao-mei and DAI Jie-jie.Morphological observation and analysis of its 18S rRNA gene of flagellate in tree shrews[J].Chinese Journal of Comparative Medicine,2015,25(11):5-8. |
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| Authors: | CHEN Ling-xi YIN Bo-wen LI Zhen-yu SUN Xiao-mei and DAI Jie-jie |
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| Institution: | Chinese Academy of Medical Sciences/Peking Union Medical College, Institute of Medical Biology, Center of Tree shrews Germplasm Resource, Yunnan Key Laboratory of major infectious diseases vaccine development, Kunming 650118, China;Chinese Academy of Medical Sciences/Peking Union Medical College, Institute of Medical Biology, Center of Tree shrews Germplasm Resource, Yunnan Key Laboratory of major infectious diseases vaccine development, Kunming 650118, China;Chinese Academy of Medical Sciences/Peking Union Medical College, Institute of Medical Biology, Center of Tree shrews Germplasm Resource, Yunnan Key Laboratory of major infectious diseases vaccine development, Kunming 650118, China;Chinese Academy of Medical Sciences/Peking Union Medical College, Institute of Medical Biology, Center of Tree shrews Germplasm Resource, Yunnan Key Laboratory of major infectious diseases vaccine development, Kunming 650118, China;Chinese Academy of Medical Sciences/Peking Union Medical College, Institute of Medical Biology, Center of Tree shrews Germplasm Resource, Yunnan Key Laboratory of major infectious diseases vaccine development, Kunming 650118, China |
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| Abstract: | Objective Morphological observation and gene analysis identification to the strain of flagellate in tree shrews.Methods The tree shrews ileocecal contents and feces by filtering centrifugal were observed microscopically direct smear and iodine staining, the total DNA extracted by the traditional method, the 18S rRNA of flagellate was amplified by PCR, then sequenced. Taking homology analysis by the BLAST, and developing the phylogenetic tree using MEGA5.1.Results Morphological observation showed that flagellate in tree shrews was tritrichomonas. Sequencing analysis indicated that homology of 18s ribosomal RNA between tree shrews giardia and the reported tritrichomonas fetus 18S ribosomal RNA(AY754332.1) was as high as 99%. Conclusion Tree shrews can affect tritrichomonas fetus and the results provide the basis for parasitology quality control of tree shrews. |
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| Keywords: | Flagellate Tree shrews Morphological observation 18S rRNA |
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