| 两种成脂诱导培养基诱导人和大鼠脂肪干细胞的研究 |
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| 引用本文： | 曹蕊,吕涛,严笠,孙雪健,肖苒.两种成脂诱导培养基诱导人和大鼠脂肪干细胞的研究[J].中国比较医学杂志,2015,25(2). |
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| 作者姓名： | 曹蕊 吕涛 严笠 孙雪健 肖苒 |
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| 作者单位： | 中国医学科学院整形外科医院,中国医学科学院整形外科医院,中国医学科学院整形外科医院,中国医学科学院整形外科医院,中国医学科学院整形外科医院 |
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| 基金项目： | 国家自然科学基金(81171817 / 30871433) |
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| 摘 要： | 目的：比较两种培养基诱导人和大鼠脂肪干细胞(Adipose-derived stem cell, ADSC)的成脂分化能力，探寻更为适宜的ADSC成脂诱导方案。方法：提取3例临床患者的脂肪干细胞(hADSCs)及出生6周大鼠的脂肪干细胞(rADSCs)。将hADSCs、rADSCs分别接种于六孔板，分为未诱导组、成脂诱导Ⅰ组和Ⅱ组，分别使用普通培养基，成脂诱导培养基Ⅰ和Ⅱ，培养10天后以油红O染色，镜下观察及检测490nm吸光度值(OD490)，比较脂滴形成情况。结果：未诱导的hADSCs和rADSCs均呈成纤维细胞样生长。成脂诱导培养3天后出现脂滴；油红O染色显示，成脂诱导组脂滴呈橘红色。hADSCs和rADSCs的成脂诱导Ⅱ组形成的脂滴均明显大于诱导Ⅰ组，统计学分析显示hADSCs的成脂诱导Ⅱ组测得的OD490明显高于诱导Ⅰ组（P<0.01); 而两组的rADSCs的OD490无明显差异（P>0.05）。结论：hADSCs和rADSCs在两种诱导培养基中均可成脂分化，但成脂诱导Ⅱ组优于成脂诱导Ⅰ组。
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| 关 键 词： | 人脂肪干细胞 大鼠脂肪干细胞 成脂诱导 |
| 收稿时间： | 2014/10/19 0:00:00 |
| 修稿时间： | 2014/11/3 0:00:00 |
Comparison of adipogenic differentiation ability of adipose stem cells derived from human beings and rats using two kinds of adipogenic medium with different components in vit |
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| CaoRui,LvTao,YanLi,SunXuejian and XiaoRan.Comparison of adipogenic differentiation ability of adipose stem cells derived from human beings and rats using two kinds of adipogenic medium with different components in vit[J].Chinese Journal of Comparative Medicine,2015,25(2). |
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| Authors: | CaoRui LvTao YanLi SunXuejian and XiaoRan |
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| Institution: | Plastic Surgery Hospital of Chinese Academy of Medical Sciences & Peking Union Medical College,Plastic Surgery Hospital of Chinese Academy of Medical Sciences & Peking Union Medical College,Plastic Surgery Hospital of Chinese Academy of Medical Sciences & Peking Union Medical College,Plastic Surgery Hospital of Chinese Academy of Medical Sciences & Peking Union Medical College,Plastic Surgery Hospital of Chinese Academy of Medical Sciences & Peking Union Medical College |
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| Abstract: | |
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| Keywords: | Human adipose derived stem cells Adipose derived stem cells of rats Adipogenic differentitation |
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