新药长毒试验动物血液生化测定规范化研究系列之一--采血部位对血液生化值的影响

目的探讨从大鼠不同部位采血对其血液生化值的影响。方法采用全自动生化仪对不同部位采血动物血液中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、总蛋白（TP）、白蛋白（ALB）、总胆红索（TBil）、碱性磷酸酶（ALP）、尿素（Urea）、肌酐（Cre）、葡萄糖（Glu）、总胆固醇（CHO）等生化值进行测定。结果（大鼠）腹主动脉及颈外静脉均与后腔静脉血液生化值在总蛋白（TP）、白蛋白（ALB）上存在明显差异，表现为前者血清中总蛋白、白蛋白明显高于后者；大鼠眶静脉与股静脉血液生化值在多项指标上亦存在明显区别。结论建议在长毒试验动物血液生化测定中，对采血部位应进行规范与统一。

Effects of Different Blood Sampling Sites on Serum Biochemical Measurement

Objective To observe the effects of different blood sampling sites on blood biochemical measurement in rats. Methods Blood samples were taken from different site of the rats. By using automatic biochemistry analyzer, a number of blood biochemical parameters were measured. Statistical analysis was performed to detect if there is significant difference among the values of blood samples taken from different sites in the rats. Results The values of some serum biochemical parameters obtained from samples taken from different sites differed significantly. The values of TP and ALB of blood taken from abdomial aorta or external jugular vein were significantly higher than those of blood taken from postcaval vein in the rats. The values of several parameters of the blood taken from orbital vein differed significantly from those from femoral vein blood samples. ConcIuslon It is important to unify the blood sampling sites in animals during chronic toxicity testing studies.