参附注射液对大鼠心肌缺血再灌注损伤的保护作用

目的探讨中成药参附注射液对在体结扎大鼠冠状动脉缺血．再灌注心肌损伤的效果以及作用机理。方法将46只SD大鼠随机分成5组，（1）非手术组；（2）结扎左冠状动脉前降支（LAD）引起心肌缺血30min对照组1；（3）心肌缺血30min给药组1；（4）心肌缺血，再灌注10min对照组2；和（5）心肌缺血，再灌注10min给药组2。分别测定心肌组织匀浆丙二醛（MDA）含量，应用透射电镜观察心肌细胞超微结构改变，并对线粒体进行体视学分析，以及抗氧化基因超氧化物岐化酶1（SOD1）和谷胱苷肽S转移酶基因的表达。结果给药组与对照组同一时间点比较心肌组织MDA含量均有显著下降（P＜0．05）；超微结构显示参附明显减小心肌细胞组织结构以及线粒体的损害；体视学分析显示对照组较非手术组线粒体有显著变化（P＜0．01），而两给药组较非手术组线粒体变化较小（P＜0．05）；参附上调SOD1和谷胱苷肽S转移酶基因的表达。结论参附注射液通过保护线粒体，减轻脂质过氧化的程度；还可通过上调SOD1及谷胱苷肽S转移酶等抗氧化基因，增加机体抗氧化能力抵抗缺血，再灌注时过氧化脂质损伤，保护心肌细胞。

Protective Effect of Shen-fu on Myocardial Ischemia-reperfusion Injury in Rats

Objective To study the protective effect and the mechanism of anti-myocardial-ischemia-reperfusion injury of Shen-fu injection through ligating the left anterior descending(LAD) of rats in vivo.Methods The 46 rats were divided into 5 groups randomLy:(1) no-operation group;(2) the control group 1: 30-minute ischemia;(3) pretreatment group 1 with Shen-fu: 30-minute ischemia;(4) the control group 2: 30-minute ischemia followed by 10-minute reperfusion;(5) pretreatment group 2 with Shen-fu: 30-minute ischemia followed by 10-minute reperfusion.The content of malondialdehyde(MDA) and the expression of antioxidant genes of superoxide dismutase 1(SOD1) gene and glutathione S-transferase gene were measured,the ultrastructure of the myocardial cells by transmission electron microscope was observed.The mitochondrial stereology analysis was done.(Results The) MDA of the pretreatment groups with Shen-fu was powful decreased compare with the control groups.The significant decreasing the lesion of the myocardial cell and mitochondrial in ultrastructure were found in the pretreatment groups with Shen-fu.The stereology analysis display that the mitochondrial in the control groups have significant changed compare with the no-operation group.And the mitochondrial in the pretreatment group with Shen-fu was a little different from the no-operation group.Shen-fu up-regulated the expression of SOD1 gene and glutathione S-transferase gene.Conclusion The Shen-fu protect the myocardial cell by reducing the lipid peroxidation.Their protective effects may be partly related to up-regulating the antioxidant gene that increase the ability of antioxidant.