Shkbp1缺失对小鼠T淋巴细胞系亚群的影响

目的探讨Shkbp1缺失小鼠(Shkbp-1~(-/-))的血液、骨髓、脾脏中血液细胞分类和T细胞亚群的变化。方法采用Shkbp-1~(-/-)转基因小鼠,经PCR鉴定基因型;利用全自动血液检测仪测定血液细胞分类;采用流式细胞仪检测Shkbp-1~(-/-)和对照组小鼠血液、骨髓、和脾脏中T淋巴细胞亚群。结果血常规结果:中性粒细胞和嗜酸性粒细胞有增高趋势,且有显著差异。淋巴细胞未见显著差异。流式结果显示:对照组血液中CD4~+CD8~+双阳性细胞降低,骨髓中CD3~+、CD4~+升高。但脾脏组织中,其CD3~+、CD4~+、CD8~+、CD4~+CD8~+双阳性细胞均呈下降趋势。结论 Shkbp1参与血液细胞的成熟分化,影响了免疫细胞数量,本研究为探索Shkbp1如何参与血液细胞的分化奠定了研究基础。

Effects of Shkbp1 deletion on mouse T lymphocyte subsets

Objective Shkbp is also called Shkbp1, can competitively inhibit binding CIN85 and c-Cbl, thereby blocking the epidermal growth factor receptor (EGFR) endocytosis and degradation, to play a role in tumor promotion. This study aims to explore the changes in blood cell classification and T cell subsets in blood, bone marrow, and spleen in Shkbp1-deletion (Shkbp-1^-/-) mice. Methods Shkbp-1^-/- transgenic mice were identified by PCR genotyping. Blood cell classification was performed using an automatic classification system. Flow cytometry was used to detect the T lymphocyte subsets in the blood, bone marrow, and spleen of Shkbp-1^-/- and control mice. Results Routine blood examination showed that neutrophils and eosinophils tended to increase and showing significant differences, and there was no significant difference in lymphocytes. The flow cytometry results showed that there was a decrease of CD4⁺CD8⁺double positive cells and increase of bone marrow CD3⁺ and CD4⁺ cells in the control group. However, there was a decreasing trend of CD3⁺, CD4⁺, CD8⁺, and CD4⁺CD8⁺cells in the spleen tissues. Conclusions Shkbp1 is involved in the maturation and differentiation of blood cells, and affects the number of immune cells. This study lays a foundation for the study of how Shkbp1 is involved in the differentiation of blood cells.