| 小鼠骨肉瘤模型构建的关键 |
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| 引用本文： | 符策岗,赵红卫,刘扬,肖运祥,陈海丹.小鼠骨肉瘤模型构建的关键[J].中国比较医学杂志,2016,26(1):73-75. |
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| 作者姓名： | 符策岗 赵红卫 刘扬 肖运祥 陈海丹 |
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| 作者单位： | 三峡大学第一临床医学院, 宜昌市中心人民医院脊柱外科/脊柱微创中心, 脊柱医学与创伤研究所, 宜昌 443000;三峡大学医学院, 宜昌 443000;三峡大学第一临床医学院, 宜昌市中心人民医院脊柱外科/脊柱微创中心, 脊柱医学与创伤研究所, 宜昌 443000;三峡大学第一临床医学院, 宜昌市中心人民医院脊柱外科/脊柱微创中心, 脊柱医学与创伤研究所, 宜昌 443000;三峡大学第一临床医学院, 宜昌市中心人民医院脊柱外科/脊柱微创中心, 脊柱医学与创伤研究所, 宜昌 443000;三峡大学第一临床医学院, 宜昌市中心人民医院脊柱外科/脊柱微创中心, 脊柱医学与创伤研究所, 宜昌 443000 |
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| 基金项目： | 国家自然科学基金(81302346);宜昌市科技研究基金(A12301-06);宜昌市中心人民医院基金(KFJ2011009)。 |
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| 摘 要： | 目的探讨骨肉瘤动物模型荷瘤成功率的关键因素。方法 24只4~8周龄C3h小鼠,其中C3h/He J型6只、C3h/He N型18只,随机分为2组。A组采用一般脱毛膏脱毛后外侧腋下注射浓度为5×10~7个数量级/m L的LM8细胞悬液0.2 m L;B组采用动物剃毛器剃毛后外侧腋下皮下注射浓度为5×10~7个数量级/m L的LM8细胞悬液0.2 m L后每日给于注射处局部按摩,并分别记录肿瘤生长情况。结果 A组成瘤率33%(4/12);B组成瘤率100%。结论不同的注射技巧对LM8的荷瘤过程及后期护理对成瘤率具有重要的意义。
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| 关 键 词： | C3h 骨肉瘤 模型 |
| 收稿时间： | 2015/10/12 0:00:00 |
| 修稿时间： | 2015/11/23 0:00:00 |
Keys in constructing osteosarcoma model of mouse |
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| FU Ce-gang,ZEN Yan,ZHAO Hong-wei,LIU Yang,XIAO Yun-xiang and CHEN Hai-dan.Keys in constructing osteosarcoma model of mouse[J].Chinese Journal of Comparative Medicine,2016,26(1):73-75. |
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| Authors: | FU Ce-gang ZEN Yan ZHAO Hong-wei LIU Yang XIAO Yun-xiang and CHEN Hai-dan |
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| Institution: | Institute of Spinal Medicine and Trauma, Department of Spinal surgery Ward/Center for Minimally Invasive Spine Surgery, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang 443000, China;Medical College of China Three Gorges University, Yichang 443000, China;Institute of Spinal Medicine and Trauma, Department of Spinal surgery Ward/Center for Minimally Invasive Spine Surgery, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang 443000, China;Institute of Spinal Medicine and Trauma, Department of Spinal surgery Ward/Center for Minimally Invasive Spine Surgery, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang 443000, China;Institute of Spinal Medicine and Trauma, Department of Spinal surgery Ward/Center for Minimally Invasive Spine Surgery, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang 443000, China;Institute of Spinal Medicine and Trauma, Department of Spinal surgery Ward/Center for Minimally Invasive Spine Surgery, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang 443000, China |
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| Abstract: | Objective Investigating the key factors in constructing animal model of osteosarcoma. Methods 24 C3h mice (C3h/HeJ type 6, C3h/HeN type 18 at 4-8 weeks old) were randomly divided into 2 groups: A group: Subcutaneous inject 0.2 mL of 5×107cells/ml mL LM8 suspension in lateral armpit before treated with depilatory creams; B group: subcutaneous inject 0.2 mL of 5×107cells/mL LM8 suspension in lateral armpit before treated with shaver, and the tumor growth was recorded. Results The rate of A was 30% (4/12), and the rate of B was 100%. Conclusions Injected techniques and the way of post handle play important role in constructing osteosarcoma model. |
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| Keywords: | C3h Osteosarcoma Models |
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