尿酸酶抑制剂在大鼠急性高尿酸血症模型复制中的应用

目的本实验考察次黄嘌呤、尿酸酶抑制剂复制大鼠代谢性高尿酸血症模型,观察造模后受试动物血清尿酸的变化,以确定尿酸酶抑制剂造模的最佳造模途径和溶剂。方法采用不同造模途径、不同溶剂给予尿酸酶抑制剂,分别测定造模后不同时段大鼠相关生化指标。结果采用灌胃给予次黄嘌呤,皮下注射以羊毛脂石蜡油为溶剂配制的尿酸酶抑制剂,可使受试动物血尿酸值明显高于空白对照组（P〈0.01）,且可维持12 h。结论以羊毛脂石蜡油（质量比3∶2）为溶剂配制尿酸酶抑制剂,采用皮下注射的方法造模,所复制的大鼠代谢性高尿酸血症模型具有血尿酸值高、维持时间长的特点。

Exploratory Development of Uricase Inhibitor for Replicating Acute Animal Model of Hyperuricemia on Rat

Objective To observe the fluctuation of uric acid in serum on mouse after replicating model by using hypoxanthine and uricase inhibitor. Make certain the most proper approach and solvent for using uricase inhibitor. Method Different approach and solvent were adopted for using uricase inhibitor, inspect the uric acid in serum at different periods of time. Results Hypoxanthine was administered via stomach, while uricase inhibitor was injected subcutaneously by lanolin mixed with olefin,which made the uric acid in serum higher than control group about 12 hours. Conclusion Uricase inhibitor was given subcutaneously by lanolin mixed with olefin in acute animal model with Hyperuricemia on rat, which has the characters of high uric acid and maintaining a long time.