血红素加氧酶-1基因在三氧化二砷诱导食管癌EC9706细胞凋亡过程中的表达

目的:检测三氧化二砷(As2O3)诱导食管癌EC9706细胞凋亡过程中血红素加氧酶-1(HO-1)基因的表达,探讨HO-1的表达与化疗药物诱导肿瘤细胞凋亡的内在联系。方法:以食管癌EC9706细胞系作为研究模型,3μmol/L AS2O3作用于EC9706细胞,镜下观察细胞形态变化;MTT法绘制细胞生长曲线;以RT-PCR方法检测HO-1mRNA的表达。结果:As2O3作用于EC9706细胞4 h即可检测到HO-1基因表达升高,12 h表达水平升至最高,24 h回落到正常水平。结论:As203诱导EC9706细胞凋亡过程中,HO-1基因表达升高,呈时间依赖性变化,可能起到短暂的对抗氧化应激作用。

The expression of HO-1gene in the apoptosis process of EC9706 cell induced by As2O3

Objective: To detect the expression of HO-1 gene in the apoptotic process of EC9706 cell induced by As2O3,and to approach the relationship between the expression of HO-1 gene and the tumor cell apoptosis induced by chemotherapeutics.Methods: EC9706 cell were treated with 3 μmol/L As2O3,then to observe the change of cell's appearance under microscope and record the cell growth curve using MTT method as well as detecting the expression of HO-1 mRNA with RT-PCR method.Results: 4 h after cells were treated with As2O3,the expression of HO-1 mRNA had begin to rise.It reached the highest level at 12 h,and at 24 h,it had return to the normal level.Conclusion: In the apoptotic process of EC9706 cell induced by As2O3,the expression of HO-1 gene rises along with the time.The change may contribute to protecting tumor cells from oxidative stress temporarily.