乙肝病毒标志物五种模式血清一氧化氮、一氧化氮合酶水平的比较

目的 :观察乙肝病毒 (HBV)感染者血清的一氧化氮 (NO)、一氧化氮合酶 (NOS)水平 ,探讨和研究NO在HBV感染过程中的变化和作用。方法 :应用ELISA法检测HBV血清标志物 ,并将HBV阳性血清分为五个常见模式组 ,应用Griess法测定各组血清NO和NOS水平与正常对照。结果 :HBsAg( +)、抗HBc( +)组 (第 4组 )和HBsAg( +)、HBeAg( +)、抗HBc( +)组 (第 5组 )显著降低 ;抗HBs( +)、抗HBc( +)组 (第 2组 )和HBsAg( +)、抗HBe( +)、抗HBc( +)组 (第 3组 ) ,NO和NOS回升 ;抗HBs( +)组 (第 1组 )和对照组水平相近。结论 :HBV在体内复制活跃时 ,可能抑制NOS活性 ,使NO合成减少 ,同时由于NO发挥对肝细胞的保护作用而使消耗增加。检测血清NO、NOS可观察HBV感染程度和预后。NO用于HBV感染的治疗有待研究

Comparisons of serum NO and NOS levels for 5 patterns of HBV marker

Objective: To observe serum NO and NOS levels in patients with HBV infection,inquire into and study NO’s change and role in HBV infecting process. Methods: HBV serum markers were detected with ELISA method;HBV positive serum were divided into 5 common pattern’s groups.Serum NO and NOS levels were detected in each group with Griess method and compared with normal controls’.Results: HBsAg(+) and anti HBc(+) in the 4’s,group as well as HBsAg(+),HBeAg(+)and anti HBc(+) in the 5’s all significantly decreased;anti HBe(+) and anti HBc(+) in the 2’s,as well as HBeAg(+),anti HBe and anti HBc(+) in the 3’s,NO and NOS gone up again;anti HBs(+) in the 1’s was similar to that in the control’s. Conclusion: When HBV was reproduced and activated in vivo,which might depress NOS activity and make NO synthesis reduce,at the same time because of NO protective effect on liver cell,NO consumption increased.Detection of serum NO and NOS could observe the degree and prognosis of HBV infection.NO in the treatment of HBV infection need to be studied.