| 2-氨基-3-苯基丙酰肼作为氨基脲敏感型胺氧化酶抑制剂的研究 |
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| 引用本文： | 林芸,罗红军,罗文鸿.2-氨基-3-苯基丙酰肼作为氨基脲敏感型胺氧化酶抑制剂的研究[J].汕头大学医学院学报,2014(1):1-4. |
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| 作者姓名： | 林芸 罗红军 罗文鸿 |
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| 作者单位： | [1]汕头大学医学院分析测试实验室 [2]汕头市中心医院药学科,广东汕头515041 |
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| 基金项目： | 国家自然科学基金资助项目（30870911）;广东省自然科学基金团队资助项目（9351507102000-001） |
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| 摘 要： | 目的：探讨人工合成2-氨基-3-苯基丙酰肼对氨基脲敏感型胺氧化酶（SSAO）的抑制效应。方法：根据文献合成2-氨基-3-苯基丙酰肼,通过核磁共振氢谱、红外光谱、质谱鉴定其结构。利用2-氨基-3-苯基丙酰肼对SSAO的抑制曲线,计算其IC50,结合透析实验确定2-氨基-3-苯基丙酰肼对SSAO抑制类型;建立高效液相色谱法测定2-氨基-3-苯基丙酰肼在水溶液中的浓度,验证其抑制类型。结果：2-氨基-3-苯基丙酰肼对SSAO的IC50为0.76μmol/L,透析后原液中2-氨基-3-苯基丙酰肼浓度为0.07μmol/L,其对SSAO的抑制率为78.0%。结论：2-氨基-3-苯基丙酰肼是SSAO不可逆抑制剂。
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| 关 键 词： | 2-氨基-3-苯基丙酰肼 氨基脲敏感型氨氧化酶 抑制效应 |
Study of 2-amino-3-phenylpropanehydrazide as Inhibitor of Semicarbazide Sensitive Amine Oxidase |
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| LIN Yun,LUO Hong-jun,LUO Wen-hong.Study of 2-amino-3-phenylpropanehydrazide as Inhibitor of Semicarbazide Sensitive Amine Oxidase[J].Journal of Shantou University Medical College,2014(1):1-4. |
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| Authors: | LIN Yun LUO Hong-jun LUO Wen-hong |
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| Institution: | 1 Analytical Instrumental Laboratory, Shantou University Medical College, Shantou 515041, China, 2 Department of Pharmacy, Shantou Central Hospital, Shantou 515041, China) |
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| Abstract: | Objective：To explore the artificial synthesis of 2-amino-3-phenylpropanehydrazide on semicarbazide sensitive amine oxidase（SSAO）inhibitory effect. Methods：2-amino-3-phenylpropanehydrazide was synthesized,and identified by IR,MS and 1H-NMR. According to the inhibition curve of 2-amino-3-phenylpropanehydrazide against SSAO,the IC50 was calculated. Dialysis experiment was performed to determine the inhibition type of 2- amino-3-phenylpropanehydrazide against SSAO. Concentration of 2-amino-3-phenylpropanehydrazide was analyzed by high performance liquid chromatography. Results：IC50 of 2-amino-3-phenylpropanehydrazide against SSAO was 0.76 μmol/L. The concentration of 2-amino-3-phenylpropanehydrazide in dialysis liquid after dialysis was 0.07 μmol/L,with inhibitory ratio of 78.0%. Conclusion：2-amino-3-phenylpropanehydrazide is an irreversible inhibitor of SSAO. |
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| Keywords: | 2-amino-3-phenylpropanehydrazide semicarbazide sensitive amine oxidase inhibitory effect |
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