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高效液相色谱法测定胆汁中头孢美唑
引用本文:李慧,罗文鸿,陈玲,张源. 高效液相色谱法测定胆汁中头孢美唑[J]. 汕头大学医学院学报, 2000, 13(2): 4-6
作者姓名:李慧  罗文鸿  陈玲  张源
作者单位:Shantou University Medical College Central Laboratory 515031  
摘    要:开发一个可用于定量测定胆汁中头孢美唑浓度的高效液相色谱分析方法。方法:取0.2mL胆汁用水稀释至1mL,并转移至已准备好的C18固相萃取柱中进行固相萃取。用1mL10%乙腈水溶液洗脱吸附在固相萃取柱上的头孢美唑,并收集于样品瓶中用高效液相色谱法进行分析测定。流劝相为20:80的乙腈:缓冲溶液(0.01molL/L磷酸盐,0.01%TBA,PH7.0)检测波长为272nm。结果:本分析方法在(1.2

关 键 词:头孢美唑 胆汁 高效液相色谱法

A High Performance Liquid Chromatographic Method for the Determination of Cefmetazole in Bile
Li Hui,Luo Wenhong,Chen Ling,Zhang Yuan. A High Performance Liquid Chromatographic Method for the Determination of Cefmetazole in Bile[J]. Journal of Shantou University Medical College, 2000, 13(2): 4-6
Authors:Li Hui  Luo Wenhong  Chen Ling  Zhang Yuan
Affiliation:Shantou University Medical College Central Laboratory 515031
Abstract:Objective:To develop an analytical method for the quantitative determination of cefmetazole in bile.Method:An aliquot of 0.2 mL bile was diluted to 1 mL with deionized water and applied onto a preconditioned C18 solid phase extraction cartridge.Cefmetazole was eluted from the cartridge with 1 mL of 10% acetonitrile in water and then analyzed with ion-paired reverse phase high performance liquid chromatography with uv detection.Mobile phase was 20∶80 acetonitrile∶buffer (0.01moL/L phosphate with 0.01%TBA,pH 7.0).The wavelength of the detector was set at 272nm.Results:The analytical method gave good linearity in the concentration range of (1.25~25)μg/mL of cefmetazole in bile(γ=0.9999).The method recoveries at different concentration levels were between 88.0%~103% with within-day and day-to-day relative standard deviations less than 5.2%.The method detection limit and quantitation limit were 1.2μg/mL and 2.4μg/mL of cefmetazole in bile,respectively.
Keywords:Cefmetazole  Bile  HPLC
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