棕榈酸诱导3T3-L1脂肪细胞肥大模型的建立

目的:探讨棕榈酸对3T3-L1脂肪细胞内甘油三酯蓄积的影响,建立肥大脂肪细胞模型.方法:分别用0.0、0.1、0.2和0.4mmol/L棕榈酸诱导成熟3T3-L1 48b,用油红O染色法鉴定脂肪细胞,用甘油三酯酶法测定胞内甘油三酯浓度,采用细胞总蛋白浓度对胞内甘油三酯浓度进行校正.结果:0.0、0.1、0.2和0.4 mmol/L棕榈酸组甘油三酯水平分别为(5.0±0.6)、(6.9±1.4)、(5.7±0.5)和(5.6±0.7)μmol/mg,0.1 mmol/L棕榈酸组可引起造模组甘油三酯的浓度增加(P＜0.05).结论:0.1 mmol/L棕榈酸能诱导成熟3T3-L1内甘油三酯蓄积.

The Establishment of Hypertrophied 3T3-L1 Adipocyte Model by Palmitate Induction

Objective: To investigate the effect of palmitate on accumulation of cytoplasmic triglyceride levels,and to establish the model of hypertrophic 3T3-L1 adipocyte.Methods: The 3T3-L1 adipocytes were treated with different concentration(0.0,0.1,0.2 and 0.4 mmol/L)of palmitate for 48 hours.The adipocytes were identified by red O staining.The levels of cytoplasmic triglyceride were determined by tissue triglyceride assay kit and adjusted by totally cytoplasmic protein concentration.Results: The levels of cytoplasmic triglyceride treated with different concentration(0.0,0.1,0.2 and 0.4 mmol/L)of palmitate were(5.0±0.6),(6.9±1.4),(5.7±0.5)and(5.6±0.7)μmol/mg respectively.Results: The 3T3-L1 adipocytes treated with of 0.1 mmol/L palmitate led to obviously accumulation of triglyceride in cytoplasm compared to the control.Conclusion: Palmitate with the concentration of 0.1 mmol/L leads to obviously accumulation of triglyceride in cytoplasm of the 3T3-L1 adipocyte.