| 紫外分光光度法测定蒙药广枣药材中总黄酮含量 |
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| 引用本文： | 吴春芝,李海华,谷福根.紫外分光光度法测定蒙药广枣药材中总黄酮含量[J].河南中医学院学报,2014(12):1786-1788. |
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| 作者姓名： | 吴春芝 李海华 谷福根 |
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| 作者单位： | 1. 内蒙古医科大学附属医院,内蒙古 呼和浩特,010050
2. 内蒙古医科大学,内蒙古 呼和浩特,010010 |
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| 基金项目： | 内蒙古医科大学附属医院科研基金项目 |
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| 摘 要： | 目的：建立蒙药材广枣中总黄酮（TFC）的含量测定方法,为完善该药材的质量标准提供依据。方法：采用紫外分光光度法,以亚硝酸钠为还原剂,硝酸铝为络合剂,氢氧化钠为显色剂,在510 nm处测定TFC的吸光度值。结果：芦丁质量在0.2～1.2 mg范围内与吸光度呈良好线性关系（r=0.999 0）,平均回收率为101.15%,RSD为0.84%（n=9）。结论：本研究所建立广枣中TFC含量测定方法,操作简便,准确可靠,可用于该药材的质量评价。
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| 关 键 词： | 广枣 总黄酮 含量测定 紫外分光光度法 |
Determination of Total Flavones from Mongolia Medicine Choerospondias axillaris by UV-Spectrophotometry |
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| Wu Chunzhi
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Li Haihua
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Gu Fugen.Determination of Total Flavones from Mongolia Medicine Choerospondias axillaris by UV-Spectrophotometry[J].Journal of Henan University of Chinese Medicine,2014(12):1786-1788. |
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| Authors: | Wu Chunzhi Li Haihua Gu Fugen |
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| Institution: | Wu Chunzhi,Li Haihua,Gu Fugen 1.Affiliated Hospital of Inner Mongolia Medical University, Hohhot Inner Mongolia China 010050 2. Inner Mongolia Medical University ,Hohhot Inner Mongolia China 010010 |
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| Abstract: | Objective： To establish a UV method for determining the content of TFC from Choerospondias axillaris so as to further provide an theoretical basis for improving the quality standards of the drug. Methods： A UV method was applied with sodium nitrite as a reductant,aluminum nitrate as complexing agent,sodium hydroxide as chromogenic agent,respectively. The absorbance of TFC was measured at 510 nm. Results： There was a good linear relationship between the absorbance and concentration of TFC ranging from 0. 2 ~ 1. 2 mg（ r = 0. 999 0） with the average recovery of 101. 15%,RSD was 0. 84%（ n = 9）. Conclusion： The established UV method is simple,accurate and reliable for controlling the quality of Choerospondias axillaris. |
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| Keywords: | Choerospondias axillaris TFC content determination UV spectrophotometry |
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