消斑固斑片对兔颈动脉粥样硬化易损斑块崩解机制的研究

目的:探讨消斑固斑片对兔颈动脉粥样硬化易损斑块崩解的机制。方法:取28-42日龄雄性SD大鼠,进行血管平滑肌细胞的原代培养,将培养细胞分为5组:空白对照组、模型组、消斑固斑片低剂量组、消斑固斑片中剂量组、消斑固斑片高剂量组。采用流式细胞术观察血管平滑肌细胞细胞间粘附分子-1蛋白表达(ICAM-1)。另高脂饲料加液氮法复制兔颈动脉粥样硬化模型,并随机分为对照组(10只),治疗组(10只),治疗组给予消斑固斑片(1.2 g/kg.d),连续灌胃15天,每天2次,两组均予高脂饮食,服药结束时。于处死动物前24 h、48 h给予中国斑点蛙蛇毒及组胺进行药物触发,以促使斑块发生实验性破裂。采用光镜观察斑块组织病理学和超微结构变化;采用酶法检测总胆固醇(TC)、三酰甘油(TG);磷钨酸-镁沉淀法检测高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)。结果:消斑固斑片有降低实验兔血中TC、TG、LDL-C,升高HDL-C的作用。同时可降低肿瘤坏死因子-α(TNF-α)诱导的ICAM-1水平及蛋白表达,且呈剂量依赖性。结论:消斑固斑片可通过降脂消斑稳定和修复易损斑块,延缓易损斑块的破裂作用。

Study on the Collapse Mechanism of the Xiaoban Guban Tablet on the Vulnerable Plaque of Rabbit Carotid Artery Sderatheroma

Objective:To investigate collapse mechanism of the Xiaoban Guban Tablet on the vulnerable plaque of rabbit carotid artery scleratheroma.Methods:4 to 6 week-old male SD rats were given primary culture of the vascular smooth muscle cells and the cultured cells were divided into five groups:blank control group,model group,Xiaoban Guban Tablet of low-dose group,Xiaoban Guban Tablet of medium-dose group,Xiaoban Guban Tablet of high-dose group.To observe the expression of intercellular adhesion molecule-1 of smoo...