| 复合PCR技术在ABO血型基因分型中的应用 |
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| 引用本文： | 刘长利,苗天红,龚晓燕,王卓妍,任芙蓉,吕秋霜,刘景汉.复合PCR技术在ABO血型基因分型中的应用[J].北京医学,2007,29(2):109-111. |
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| 作者姓名： | 刘长利 苗天红 龚晓燕 王卓妍 任芙蓉 吕秋霜 刘景汉 |
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| 作者单位： | 解放军总医院输血科,100853;北京红十字血液中心 |
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| 摘 要： | 目的 探讨复合PCR技术在ABO血型基因分型方法中的应用.方法 通过实验研究摸索复合PCR的条件和规律,采用单管同时扩增出ABO基因的第6、7外显子后,使用限制性内切酶对复合PCR产物进行酶切消化.结果 通过最佳实验反应条件的获得,可建立ABO血型基因分型方法.结论 复合PCR技术在建立ABO血型基因分型方法的应用中,具有其特殊性.
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| 关 键 词： | ABO血型 基因分型 复合PCR |
| 修稿时间： | 2006-05-17 |
Application of multiplex PCR on ABO genotyping |
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| LIU Chang-li, MIAO Tian-hong, GONG Xiao-yan, et al.Application of multiplex PCR on ABO genotyping[J].Beijing Medical Journal,2007,29(2):109-111. |
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| Authors: | LIU Chang-li MIAO Tian-hong GONG Xiao-yan |
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| Institution: | Department of Blood Transfusion, General Hospital of PLA, Beijing 100853 |
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| Abstract: | Objective To establish a rapid ABO genotyping method by using muhiplex-PCR technique. Methods The optimal condition of muhplex-PCR was determined through trials and errors, then the amplification products were digested with two restriction enzymes. Results After the optimal experimental conditions were determined, the ABO genotyping method was established. Conclusions The application of multiplex PCR on ABO genotyping has its unique features. |
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| Keywords: | ABO blood group Genotype Muhiplex-PCR |
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