| FLOW CYTOMETRIC ANALYSIS OF DNA CONTENT FOR EARLY DETECTION, ESTIMATING PROGNOSIS IN BLADDER CANCER |
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| 作者姓名： | 凌春华 叶敏 蒋鹤鸣 安世源 刘炳荣 |
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| 作者单位： | Department of Urology，Xinhua Hospital，SSMU，Shanghai，Department of Urology，Xinhua Hospital，SSMU，Shanghai，Department of Urology，Xinhua Hospital，SSMU，Shanghai，Department of Urology，Xinhua Hospital，SSMU，Shanghai，Department of Biophysics，SSMU，Shanghai |
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| 摘 要： | Flow cytometry (FCM) was used to analyze the DNA content of epithelial cells in 26 cases of bladder cancer and 10 cases of nonneoplastic disease. Bladder tumor cells can be identified by aneuploidy or hyperdiploidy in histogram and/or a heterogeneity index (HI) greater than 2.30. The percentage of positive FCM in patients with bladder cancer was 84.62%. With respect to histological grading, it was 77.78% in grade 1, 90.91% in grade 2 and 100% in grade 3. While in nonneoplastic cases it was 10% Urinary exfoliative cytology was examined in all 26 patients with bladder cancer, with an accuracy of 61.54%. The DNA content of tumor cells increased with the increase of tumor grade and stage. Follow up showed that of 22 bladder cancer patients treated conservatively by transurethral resection or partial cystectomy, 8 recurred. The HI value in recurrent cases was higher than that in nonrecurrent cases. Among these 8 patients, 6 were correctly predicted by FCM. FCM appears to be an objective, sensitive and quantitative method for the diagnosis and monitoring of bladder cancer, and may be useful in estimating the prognosis of superficial bladder cancer.
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FLOW CYTOMETRIC ANALYSIS OF DNA CONTENT FOR EARLY DETECTION, ESTIMATING PROGNOSIS IN BLADDER CANCER |
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| Ling Chunhua,Ye Min Jiang Heming An Shiyuan.FLOW CYTOMETRIC ANALYSIS OF DNA CONTENT FOR EARLY DETECTION, ESTIMATING PROGNOSIS IN BLADDER CANCER[J].Journal of Shanghai Second Medical University(Foreign Language Edition),1989(Z1). |
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| Authors: | Ling Chunhua Ye Min Jiang Heming An Shiyuan |
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| Institution: | Ling Chunhua,Ye Min Jiang Heming An Shiyuan Department of Urology,Xinhua Hospital,SSMU,Shanghai Liu Bingrong Department of Biophysics,SSMU,Shanghai |
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| Abstract: | Flow cytometry (FCM) was used to analyze the DNA content of epithelial cells in 26 cases of bladder cancer and 10 cases of nonneoplastic disease. Bladder tumor cells can be identified by aneuploidy or hyperdiploidy in histogram and/or a heterogeneity index (HI) greater than 2.30. The percentage of positive FCM in patients with bladder cancer was 84.62%. With respect to histological grading, it was 77.78% in grade 1, 90.91% in grade 2 and 100% in grade 3. While in nonneoplastic cases it was 10% Urinary exfoliative cytology was examined in all 26 patients with bladder cancer, with an accuracy of 61.54%. The DNA content of tumor cells increased with the increase of tumor grade and stage. Follow up showed that of 22 bladder cancer patients treated conservatively by transurethral resection or partial cystectomy, 8 recurred. The HI value in recurrent cases was higher than that in nonrecurrent cases. Among these 8 patients, 6 were correctly predicted by FCM. FCM appears to be an objective, sensitive and quantitative method for the diagnosis and monitoring of bladder cancer, and may be useful in estimating the prognosis of superficial bladder cancer. |
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| Keywords: | flow cytometry bladder cancer DNA |
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