| 源于纤维单胞菌的聚阿拉伯糖内切酶性质的研究 |
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| 引用本文： | 张晓影,辛毅.源于纤维单胞菌的聚阿拉伯糖内切酶性质的研究[J].西部医学,2008,20(5):919-921. |
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| 作者姓名： | 张晓影 辛毅 |
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| 作者单位： | 大连医科大学,辽宁大连116044 |
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| 摘 要： | 目的确定源于纤维单胞菌（Cellulomonose sp．）的聚阿拉伯糖内切酶的性质。方法SDS—PAGE和凝胶层析测定相对分子质量，离子交换层析估计等电点，TLC法测定酶活力，Lineweare—Burk双倒数做图法测定酶促反应动力学特性。结果相对分子质量为45kD，等电点约为6．0，酶促反应动力学符合Michaelis—Menten动力学规律，在37℃反应条件下，米氏常数Km为2．5mg／ml，最大反应速率Vmax为0．083μmol／L·min，最适pH为8．0，pH6．0～9．0时较稳定。最适温度为40℃，在40℃以下较稳定，超过50℃时酶活力开始下降。结论确定了一种聚阿拉伯糖内切酶的性质。
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| 关 键 词： | 聚阿拉伯糖 六聚阿拉伯糖 聚阿拉伯糖内切酶 |
Characterization of endo-arabinase secreted from Cellulomonose |
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| ZHANG Xiao-ying,XIN Yi.Characterization of endo-arabinase secreted from Cellulomonose[J].Medical Journal of West China,2008,20(5):919-921. |
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| Authors: | ZHANG Xiao-ying XIN Yi |
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| Institution: | ( Dalian Medical University, Dalian 116044, Liaoning , China) |
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| Abstract: | Objective To investigate the properties of endo-arabinase secreted from Cellulomonose. Methods The molecular weight was determined with SDS-PAGE and gel filtration. The isoelectric point was measured with anion exchange. The activity of endo-arabinase was detected with TLC assay, and enzyme kinetic parameters with Linewear- Burk. Results The molecular weight of endo-arabinase is 45kD and the isoelectric point is 6. 0. The apparent Km values was 2.5 mg/ml and apparent Vmax was 0. 083μmol/（L · min）. Conclusion The properties of endo-arabinase secreted from Cellulomonose is confirmed. |
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| Keywords: | Arabinogalactan Hexa-arabinofuranoside Endo-arabinase |
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