慢性心衰患者 Th1与 Th2型细胞及 Treg细胞的表达

目的探讨Th1、Th2型细胞及Treg细胞在慢性心衰发病中的作用机制。方法采用RT-PCR技术检测62例慢性心衰患者和42例正常人外周血单个核细胞中T—bet、GATA-3及Foxp3mRNA的表达水平；ELISA法检测血浆中Th1／Th2型细胞因子IFN-γ／IL-4水平。结果慢性心衰患者T-bet表达水平高于正常对照组，GATA-3及Foxp3表达水平低于正常对照组（均P〈0．05）。慢性心衰组患者血浆中IFN-γ水平及IFN-γ／IL-4高于对照组（均P〈0．01）。结论慢性心衰患者T-bet／GATA-3及IFN-γ／IL-4升高，Foxp3降低，提示Th1细胞亚群表达升高，Th2型细胞及CD4＋、CD25＋调节性T细胞减少，细胞免疫异常可能参与慢性心衰的发病机制。

Research on the expression of Th1, Th2 and Treg cells in patients with chronic heart failure

Objective To explore the roles of Thl, Th2 and Treg cells in pathogenesis of chronic heart failure （CHF）. Methods Real-time PCR was employed to detect the expression of T-bet,GATA-3 and Foxp3 mRNA in pe- ripheral blood mononuclear cells from 62 patients with CHF and 42 healthy controls. ELISA was used to detect serum Th1/Th2 cytokines IFN-γ/IL-4. Results The expression level of T-bet mRNA in the CHF group was higher than that in the control group （P〈0.05）. The expression levels of GATA-3 mRNA and Foxp3 mRNA in the CHF group was lower than that in the control group（P〈0. 05）. The expression levels of IFN-γ/ and IFN-γ/IL-4 in the CHF group was higher than that in the control group（ P〈0. 01）. Conclusion The ratios of T-bet/GATA-3 and IFN-γ/IL-4 increases in patients with CHF, while Foxp3 decreases show that the expression of Th1 cells is higher, and expression of Th2 and Treg is lower. There is a high possibility that the abnormality of cellular immunity may play an important role in the pathogenesis of CHF.