五倍子酸对小鼠胃癌MFC和肝癌H22细胞增殖的抑制作用

目的:研究五倍子酸(GA)对小鼠胃癌MFC细胞及小鼠肝癌H22细胞增殖的影响。方法:分别用3.125、6.250、12.500、25.000、50.000和100.000mg/L的GA处理MFC和H22细胞48h后,应用MTT法检测细胞的增殖抑制率;分别以6.25、12.50和25.00mg/L的GA作用MFC和H22细胞48h后,应用流式细胞仪检测细胞周期及凋亡情况;建立移植性H22和MFC荷瘤小鼠模型,分别给予0.50、0.25和0.20g/kgGA、环磷酰和生理盐水10d后计算抑瘤率。结果:GA呈剂量依赖性抑制MFC及H22细胞增殖(F=70.845、145.444,P<0.001)。GA使MFC细胞和H22细胞均阻滞在G0/G1期(F=80.432、42.903,P<0.001),各组MFC细胞凋亡率差异有统计学意义(F=45.831,P<0.001)。不同剂量GA对荷MFC和H22瘤小鼠的抑瘤率差异有统计学意义(F=206.264、110.906,P<0.001)。结论:GA具有较好的抑制MFC和H22细胞增殖的作用。

Inhibitive effects of gallic acid on proliferation of murine gastric cancer and hepatoma-22 cells

Aim:To explore the effect of gallic acid(GA) on proliferation of murine gastric cancer(MFC) and hepatoma-22(H22) cells.Methods:MTT was employed to detect proliferation ability of MFC and H22 cells treated with 3.125,6.250,12.500,25.000,50.000 and 100.000 mg/L GA for 48 h,respectively.Flow cytometry was used to examine cell cycle progression and apoptotic rate of MFC and H22 cells treated with 6.25,12.50 and 25.00 mg/L GA for 48 h.Tumor inhibition rates were calculated after the mice bearing H22 or MFC cells,which were administered GA(0.50,0.25 and 0.20 g/kg),cyclophosphamide or normal saline for 10 days.Results:The proliferation inhibtion rate of GA on MFC and H22 cells were enhanced in a dose-dependant manner(F=70.845,145.444;P<0.001).Both MFC and H22 cells were arrested at G0/G1(F=80.432,42.903;P<0.001) and increase of cellular apoptosis was detected in MFC cells(F=45.831,P<0.001).The anti-tumor rates of the different dosage of GA for mice bearing MFC and H22 cells had significant differences(F=206.264,110.906;P<0.001).Conclusion:GA is applicable to inhibit proliferation of MFC and H22 cells both in vivo and in vitro mainly.