核酸扩增-液相杂交法检测沙眼衣原体DNA

目的：探讨用核酸扩增（PCR）－液相杂交法检测沙眼衣原体DNA的效果。方法：用PCR－液相杂交法对616份临床标本进行了沙眼衣原体检测，并与培养法比较，对与培养法结果不相符合的标本及部分阴性标本进行连接酶链反应（LCR）复检。结果：PCR－液相杂交相法检测沙眼衣原体特异性良好。灵敏度达到0.1fg，临床标本沙眼衣原体DNA阳性检出率为27.6％，显著高于培养法的6.3%（P＜0.001）。以培养法为标准，此法的灵敏度为100％。以LCR法为标准，此法的灵敏度为97.2％,特异度为80.7%，2者具有较好的相符性。结论：PCR－液相杂交法检测沙眼衣原体DNA操作简便，特异性强，灵敏度高，适合临床应用。

Detection of the chlamydia trachomatis DNA by PCR-liquid hybridization method

Aim: To study the method of detecting chlamydia trachomatis (CT) DNA by PCR-liquid hybridization. Meth-ods: A total of 616 urogenital specimens from sexually transmitted disease (STD) clinics were subjected to CT DNA detection by PCR-liquid hybridization method, and the result was compared with that of the cell culture method. For the specimens which did not accord with culture result and the results were negative, the LCR method was applied. Results: PCR method had a higher specificity for detecting CT DNA, and could detect 0. 1 fg of CT DNA. The positivity rate (27.6%) of PCR was significantly higher than that of cell culture(6. 3% , P < 0. 01) . When cell cultrue was used as the standard, the sensitivity of PCR was100% . Compared with LCR, the sensitivity and specificity of PCR method were 97.2% and 80.7% , respectively. Conclusion: PCR is a simple, sensitive, and specific method to detect CT DNA , and it is suitable for clinical use.