| 恶性黑色素瘤淋巴道转移裸小鼠模型的建立 |
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| 引用本文： | 张沛,刘进忠.恶性黑色素瘤淋巴道转移裸小鼠模型的建立[J].郑州大学学报(医学版),2011,46(1):92-94. |
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| 作者姓名： | 张沛 刘进忠 |
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| 作者单位： | 郑州大学口腔医学院病理科,郑州,450052 |
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| 摘 要： | 目的:建立恶性黑色素瘤淋巴道转移裸小鼠模型.方法:12只裸小鼠分为2组,每组6只.实验组通过裸小鼠角膜囊袋植入恶性黑色素瘤A375细胞建立恶性黑色素瘤淋巴道转移裸小鼠模型,对照组未植入瘤细胞.2周后取材,进行淋巴内皮细胞特异性抗体LYVE-1角膜全组织荧光染色,采用图像分析法测定角膜淋巴管总体面积,RT-PCR法检测角...
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| 关 键 词： | 恶性黑色素瘤 淋巴道转移 血管内皮生长因子C 淋巴管再生 裸鼠 |
Establishment of malignant melanoma lymphatic metastasis model in nude mice |
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| ZHANG Pei
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LIU Jinzhong.Establishment of malignant melanoma lymphatic metastasis model in nude mice[J].Journal of Zhengzhou University: Med Sci,2011,46(1):92-94. |
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| Authors: | ZHANG Pei LIU Jinzhong |
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| Institution: | ZHANG Pei,LIU Jinzhong Department of Pathology,College of Stomatology,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim:To establish a nude mice model of malignant melanoma lymphatic metastasis.Methods:The mice in experimental group(n=6)were implanted malignant melanoma A373 cells combined with matrigel into the cornea.The mice in control group(n=6)were given PBS combined with matrigel into the cornea.After 2 weeks,the immunorescent whole mount staining for lymphatic specific marker LYVE-1 was performed,and RT-PCR was used to detect the expression of vascular endothelial growth factor-C(VEGF-C)mRNA.Results:The total area... |
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| Keywords: | malignant melanoma lymphatic metastasis vascular endothelial growth factor-C lymphangiogenesis nude mouse |
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