| 人B7-2基因修饰的食管癌细胞瘤苗抗肿瘤的免疫效应 |
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| 引用本文： | 赵国强,汤黎明,路静,赵军,宋谦,崔自由,杨洪艳,赵继敏,黄幼田,董子明.人B7-2基因修饰的食管癌细胞瘤苗抗肿瘤的免疫效应[J].郑州大学学报(医学版),2005,40(4):588-590. |
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| 作者姓名： | 赵国强 汤黎明 路静 赵军 宋谦 崔自由 杨洪艳 赵继敏 黄幼田 董子明 |
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| 作者单位： | 1. 郑州大学基础医学院微生物学与免疫学教研室,郑州,450052
2. 郑州大学基础医学院病理生理学教研室,郑州,450052 |
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| 基金项目： | 河南省科技攻关基金资助项目0324410034，河南省科技创新人才工程项目20008402 |
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| 摘 要： | 目的:研究人B7-2基因修饰的食管癌细胞作为瘤苗的抗肿瘤作用。方法:将真核荧光表达载体pEGFP-C3-B7-2,通过脂质体转染技术转染人食管癌细胞株EC9706。外周血来源的树突状细胞(DC)负载肿瘤抗原后,与自体T淋巴细胞共培养3d,获得细胞毒性T淋巴细胞(CTL)。用四甲基偶氮唑蓝(MTT)法检测CTL对转染和未转染pEGFP-C3-B7-2的人食管癌细胞EC9706的杀伤活性。结果:CTL对转染pEGFP-C3-B7-2的肿瘤细胞的杀伤活性大于转染pEGFP-C3和未转染细胞的杀伤活性(P<0.05)。结论:人B7-2基因修饰的EC9706肿瘤细胞瘤苗,可诱导出明显的抗EC9706细胞的免疫效应。
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| 关 键 词： | 人B7-2 绿色荧光蛋白 肿瘤疫苗 免疫效应 |
| 修稿时间： | 2005年3月10日 |
Antitumor immunological effect of human B7-2 gene modified by esophageal cancer cell vaccine |
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| ZHAO Guoqiang,TANG Liming,LU Jing,ZHAO Jun,SONG Qian,CUI Ziyou,Yang Hongyan,Zhao Jimin,Huang Youtian,DONG Ziming.Antitumor immunological effect of human B7-2 gene modified by esophageal cancer cell vaccine[J].Journal of Zhengzhou University: Med Sci,2005,40(4):588-590. |
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| Authors: | ZHAO Guoqiang TANG Liming LU Jing ZHAO Jun SONG Qian CUI Ziyou Yang Hongyan Zhao Jimin Huang Youtian DONG Ziming |
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| Institution: | ZHAO Guoqiang 1),TANG Liming 2),LU Jing 2),ZHAO Jun 2),SONG Qian 2),CUI Ziyou 2),YANG Hongyan 2),ZHAO Jimin 2),HUANG Youtian 2),DONG Ziming 2) 1)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 2)Department of Pathophysiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim: To study the immunological effect of esophageal cancer cell vaccine modified by human B7-2 gene. Methods: Human esophageal cancer cell line EC9706 was transfected with the vector of pEGFP-C3-B7-2 uging the technique of lipofectamine transfection. The dendritic cells(DCs) induced from peripheral blood mononuclear cells(PBMC) were loaded with the tumor antigen, and co-cultured with congeneric T cells derived from PBMC for 3 days,to obtain the tumor specific cytotoxic T lymphocytes(CTL). Methyl thiazolyl tetrazolium(MTT) assay was used to detect inhibition effect of CTL on transfected and untransfected EC9706 cells. Results: CTL had stronger inhibition response to the cancer cells transfected with pEGFP-C3-B7 GAAB2 2 than to the cancer cells transfected with pEGFP-C3 and the untransfected cancer cells (P< 0.05).Conclusion: The tumor vaccine modified by human B7-2 gene can significantly induce response to kill esophageal cancer cells. |
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| Keywords: | human B7-2 green fluorescent protein tumor vaccine immunological effect |
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