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| 8-Br-cAMP和槲皮素对Eca-109细胞周期及凋亡的影响 | |
| 引用本文： | 任伟宏,宫璀璀,吴景兰,赵素玲,赵志娟,苏安英.8-Br-cAMP和槲皮素对Eca-109细胞周期及凋亡的影响[J].郑州大学学报(医学版),2003,38(5):678-681. |
| 作者姓名： | 任伟宏 宫璀璀 吴景兰 赵素玲 赵志娟 苏安英 |
| 作者单位： | 1. 河南中医学院第一附属医院检验科,郑州,450000 2. 解放军第153医院检验中心,郑州,450042 3. 郑州大学分子细胞生物研究中心,郑州,450052 4. 河北工程学院医学部,邯郸,056029 |
| 基金项目： | 河南省自然科学基金资助项目 984021800;河南省教育科研基金资助项目 200018007 |
| 摘 要： | 目的观察8-Br-cAMP和槲皮素(quercetin)对Eca-109细胞周期及凋亡的影响.方法将贴壁培养的Eca-109细胞随机分成3组,Br组加8-Br-cAMP至终浓度为2×105 mol/L;Q组加槲皮素至终浓度为43 μmol/L;C组不加任何药物,只加入新培养液培养.3组在同等条件下继续培养48 h.采用流式细胞仪检测细胞的周期,采用TUNEL法检测细胞凋亡百分率,采用DNA琼脂糖凝胶电泳法观察细胞DNA降解情况.结果2实验组细胞中G2-M期细胞比例较对照组显著上升(P<0.01).细胞凋亡百分率2实验组显著高于对照组(P<0.01);Br组和Q组相比,差异无统计学意义(P>0.05).DNA琼脂糖凝胶电泳中Br组和Q组皆呈DNA"梯样型"带,对照组未呈现DNA降解.结论8-Br-cAMP和槲皮素皆可阻滞Eca-109细胞于G2-M期,并进一步诱导Eca-109细胞凋亡. |
| 关 键 词： | 细胞凋亡 8-Br-cAMP 槲皮素 Eca-109细胞 食管癌 |
| 修稿时间： | 2003年2月20日 |
Effects of 8-Br-cAMP and quercetin on the cell cycle and cell apoptosis in Eca-109 cells |
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| REN Weihong ,GONG Cuicui ,WU Jinglan ,ZHAO Suling ,ZHAO Zhijuan ,SU Anying.Effects of 8-Br-cAMP and quercetin on the cell cycle and cell apoptosis in Eca-109 cells[J].Journal of Zhengzhou University: Med Sci,2003,38(5):678-681. | |
| Authors: | REN Weihong GONG Cuicui WU Jinglan ZHAO Suling ZHAO Zhijuan SU Anying |
| Institution: | REN Weihong 1),GONG Cuicui 2),WU Jinglan 3),ZHAO Suling 1),ZHAO Zhijuan 1),SU Anying 4) 1)Department of Clinical Laboratory,the First Affiliated Hospital,Henan Traditional Chinese Medicine College,Zhengzhou 450000 2)Laboratory Medicine Center,the PLA No.153 Hospital,Zhengzhou 450042 3)Molecular Cell Biology Research Center,Zhengzhou University,Zhengzhou 450052 4)Medical Center,Hebei Engineering College,Handan 056029 |
| Abstract: | Aim:To study the effects of 8 Br cAMP and quercetin on the cell cycle and apoptosis in human esophageal cancer Eca 109 cells. Methods: The cultured Eca 109 cells were randomly divided into 3 groups: 8 Br cAMP (Br)group, Quercetin (Q)group, and Control (C)group with no any drugs.The cells of 3 groups were cultured for 48 h under the same condition.The cell cycle was detected by flow cytometry, "DNA ladder" was detected with agarose gelelectrophoresis, The cell apoptosis percentage was demonstrated by TUNEL.Results: Compared with the control group the cell percentage of G 2 M phase in Br group and Q group was enhanced ( P <0.01);the cell apoptosis percentage in Br or Q group was increased significantly in comparision with that of the control group( P <0.01),while there was no significant difference between Br and Q groups( P >0.05);the DNA "ladder pattern" showed in Br and Q groups,whereas no DNA degradation showed in the control group. Conclusion: The Eca 109 cells could be blocked in G 2 M phase by 8 Br cAMP and quercetin, and further result in cell apoptosis. |
| Keywords: | apoptosis 8 Br cAMP quercetin Eca 109 cell line |
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