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大鼠哮喘模型气道重塑中细胞增殖和凋亡的变化
引用本文:贾天明,乔俊英,栾斌,邹丽萍,冯剑飞.大鼠哮喘模型气道重塑中细胞增殖和凋亡的变化[J].郑州大学学报(医学版),2004,39(3):449-452.
作者姓名:贾天明  乔俊英  栾斌  邹丽萍  冯剑飞
作者单位:郑州大学第三附属医院儿科,郑州,450052
摘    要:目的探讨大鼠哮喘气道重塑中细胞增殖和凋亡的变化,以及地塞米松对其影响.方法将39只大鼠随机等分为正常对照组、哮喘模型组和地塞米松干预组,以卵蛋白致敏制作大鼠哮喘模型,病理观察3组气道壁形态学改变;采用免疫组化方法观察细胞增殖核抗原(PCNA)和Caspase-3在气道壁和肺组织中的表达.结果哮喘模型组气道壁、气道周围及肺泡区细胞PCNA阳性表达(13.00±1.87,20.46±4.16)明显高于正常对照组的(10.00±2.20)及(15.00±4.83)(P<0.05),且与气道上皮层、黏膜层厚度及胶原沉积面积呈正相关(r=0.67,0.66,0.57;P<0.05).地塞米松干预组PCNA表达(9.54±2.15,13.08±3.40)明显低于哮喘模型组,Caspase-3表达(5.38±1.45,3.82±1.70)高于哮喘模型组(4.92±1,85,5.62±1.56)(P<0.05).结论细胞增殖过度和凋亡异常是哮喘气道炎症和重塑反应的重要机制之一,地塞米松可通过调控细胞增殖和凋亡阻止气道重塑发生.

关 键 词:哮喘  气道重塑  细胞增殖  凋亡  地塞米松  大鼠
修稿时间:2003年11月28

Changes of cell proliferation and apoptosis in airway remodeling of experimental asthmatic rats
JIA Tianming,QIAO Junying,LUAN Bin,ZOU Liping,FENG Jianfei.Changes of cell proliferation and apoptosis in airway remodeling of experimental asthmatic rats[J].Journal of Zhengzhou University: Med Sci,2004,39(3):449-452.
Authors:JIA Tianming  QIAO Junying  LUAN Bin  ZOU Liping  FENG Jianfei
Institution:JIA Tianming,QIAO Junying,LUAN Bin,ZOU Liping,FENG JianfeiDepartment of Pediatrics,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
Abstract:Aim:To explore the change of cell proliferatation and apoptosis in airway remodeling of asthmatic rats and the effect of dexamesone. Methods: SD rat were divided into normal group,model group and dexamesone group.Asthmatic rat models were established by repeated ovalbumin sensition. The morphological changes of airway were observed; the expression of PCNA and Caspase 3 was estimated by immunohistochemical technique. Results: The expression of PCNA in model group were higher than those of normal and dexamesone groups,and positive correlated with the depth of airway epithelia and mucosa layers and collagen deposit area. The expression of Caspase 3 in dexamesone group was higher than that of model group. Conclusion:The disorder of cell proliferation and apoptosis is one of the important mechanisms of airway inflammation and remodeling in asthma, and dexamesone can prevent airway remodeling by regulating the cell proliferation and apoptosis.
Keywords:asthma  airway remodeling  cell proliferation  apoptosis  dexameasone
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