肺癌患者CYP1A1和GSTM1基因多态性检测

目的:探讨CYP1A1与GSTM1基因多态与支气管肺癌癌变的关系.方法:采用回顾性"病例-对照"方法和PCR-RFLP技术,对98例肺癌患者和136名体检健康者(对照组)进行CYP1A1与GSTM1基因多态性检测.结果:对照组和肺癌组CYP1A1 m1、GSTM1缺陷型等位基因频率分别为28%和43%、44%和61%,2组比较,差异有统计学意义(P＜0.05).CYP1A1(w1/m1、CYP1A1(m1/m1、GSTM1(缺陷型)基因型患肺癌的危险度分别升高3.18倍、2.72倍和2.16倍(P均＜0.05).GSTM1(缺陷型)和CYP1A1(w1/m1或CYP1A1(m1/m1基因型携带者患肺癌的危险度为5.62倍(P＜0.01.吸烟使GSTM1缺陷型携带者和CYP1A1 m1携带者肺癌的患病危险度较单一基因作用危险度显著增加(P＜0.05).结论:CYP1A1 m1和GSTM1缺陷型基因均是肺癌的危险因素,2者存在交互作用,且均与吸烟有协同作用.

CYP1A1 and GSTM1 polymorphisms and susceptibility to lung cancer

Aim: To study the relationship between the cytochrome P-450 1A1(CYP1A1) and glutathione S-transferase M1(GSTM1) and the occurrence of bronchic lung cancer(LC). Methods:The genetic polymorphism of CYP1A1 and GSTM1 were detected in 98 cases of lung cancer and 136 healthy controls using case-control study and PCR-RFLP. Results: For CYP1A1, the allele frequencies of m1 were 28% and 44%, for GSTM1, the allele frequencies of deletion were 43% and 61% in control and LC groups, respectively. OR for the CYP1A1 ((w1/m1) or (w1/m1)) genotype, GSTM1 deletion genotype indicated 3.18, 2.72 and 2.16 fold increased risk for developing LC. Individuals with combination of the GSTM1(deletion)/CYP1A1 ((w1/m1) or (m1/m1)) genotypes indicated 5.62 fold increased risk for developing LC. CYP1A1 ((w1/m1) or (w1/m1)), GSTM1 deletion genotypes and smoking increased risk for developing LC, significantly.Conclusion: The mutant genotypes of the CYP1A1 m1 and GSTM1 deletion polymorphisms raise risk of LC,and the two genetic polymorphisms and smoking have the joint effect.