| DTT与顺铂诱导对食管癌Eca109细胞磷酸化P38表达的影响 |
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| 引用本文： | 冯若,翟文龙,刘国红,金辉,张钦宪.DTT与顺铂诱导对食管癌Eca109细胞磷酸化P38表达的影响[J].郑州大学学报(医学版),2007,42(5):893-895. |
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| 作者姓名： | 冯若 翟文龙 刘国红 金辉 张钦宪 |
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| 作者单位： | 1. 郑州大学基础医学院组织学与胚胎学教研室,郑州,450001
2. 郑州大学第一附属医院普外科,郑州,450052 |
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| 摘 要： | 目的:探讨磷酸化P38在顺铂和DTT诱导的食管癌Eca109细胞凋亡中的作用.方法:分别用DTT与顺铂诱导处理食管癌Eca109细胞,未加药组作为对照.采用流式细胞仪技术检测细胞凋亡率;用免疫组化方法检测磷酸化P38的表达情况.结果:与对照组相比,DTT处理组及顺铂处理组细胞凋亡率明显升高.免疫组化结果显示:2处理组磷酸化P38的水平均明显高于对照组(P＜0.05);DTT处理组磷酸化P38大部分位于细胞核内,而顺铂组磷酸化P38则主要弥散于细胞浆内.结论:磷酸化P38在DTT和顺铂诱导的食管癌Eca109细胞凋亡时细胞内分布不同,激活的P38可能通过不同途径参与细胞凋亡.
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| 关 键 词： | P38 凋亡 顺铂 DTT Eca109细胞系 |
| 修稿时间： | 2007-01-22 |
Relationship between the expression of p-P38 and apoptosis of Eca109 cells induced by cisplatin and DTT |
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| FENG Ruo,ZHAI Wenlong,LIU Guohong,JIN Hui,ZHANG Qinxian.Relationship between the expression of p-P38 and apoptosis of Eca109 cells induced by cisplatin and DTT[J].Journal of Zhengzhou University: Med Sci,2007,42(5):893-895. |
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| Authors: | FENG Ruo ZHAI Wenlong LIU Guohong JIN Hui ZHANG Qinxian |
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| Institution: | 1 Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001 ;2 Department of General Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 |
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| Abstract: | Aim:To study the relationship between the expression of phosphorylated P38(p-P38) and apoptosis of Eca109 cells induced by cisplatin and DTT.Methods:Esophageal carcinoma Eca109 cells were treated with either cisplatin or DTT.The untreated cells served as the control.The cell apoptosis was detected by flow cytometry.The P38 phosphorylation was detected by immunohistochemistry.Results:The apoptotic rates of Eca109 cells in cisplatin and DTT treatment groups were higher than that of control group(P<0.05).The phosphorylation level of P38 in the 2 treatment groups was much higher than that of control group(P<0.05).DTT treatment,but not cisplatin treatment,induced nuclear translocation of p-P38.Conclusion:The localization of p-P38 is different in Eca109 cell apoptosis induced by cisplatin and DTT.P38 activation may be associated with multiple apoptosis pathways. |
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| Keywords: | P38 apoptosis cisplatin DTT Eca109 cell line |
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